ArticlePloS one2016
Proteomic Characterization of Middle Ear Fluid Confirms Neutrophil Extracellular Traps as a Predominant Innate Immune Response in Chronic Otitis Media.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
What it found
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Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it, 45 citations in OpenAlex.
- Dysregulation of immune response in otitis media.Expert reviews in molecular medicine · 2021Pooled it
- The emerging role of immunothrombosis in paediatric conditions.Pediatric research · 2019Pooled it
- Type I interferon signaling in hematopoietic cells impairs neutrophil antibacterial function in the middle ear during viral co-infection.Cell reports. Medicine · 2026Article
- Higher levels of neutrophil extracellular traps in mucoid middle ear effusion obtained from otitis media with effusion in children.Annals of medicine · 2025Article
- Blood Inflammatory Markers as Predictors of Effusion Characteristics and Postoperative Hearing Outcomes in Children with Otitis Media with Effusion: A Retrospective Study.Medicina (Kaunas, Lithuania) · 2025Article
- Saikosaponin D inhibits the inflammatory response of secretory otitis media through FTO-mediated NBMC pharmacology & toxicology · 2025Article
- Exploiting haem-iron dependence of nontypeableFrontiers in cellular and infection microbiology · 2025Review
- Establishment of novel immortalized middle ear cell lines as models for otitis media.Laryngoscope investigative otolaryngology · 2023Article
- Acute organ injury and long-term sequelae of severe pneumococcal infections.Pneumonia (Nathan Qld.) · 2023Review
- Trans-cortical vessels in the mouse temporal bulla bone are a means to recruit myeloid cells in chronic otitis media and limit peripheral leukogram changes.Frontiers in genetics · 2022Article
- Gene expression analysis reveals immune and metabolic candidate pathways in the pathogenesis of chronic otitis media.American journal of translational research · 2022Article
- Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion.Frontiers in pediatrics · 2021Article
- Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media.International journal of molecular sciences · 2020Article
- Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs.Journal of veterinary internal medicine · 2020Article
- MUC5B induces in vitro neutrophil extracellular trap formation: Implication in otitis media.Laryngoscope investigative otolaryngology · 2020Article
- Panel 2- recent advance in otitis media bioinformatics.International journal of pediatric otorhinolaryngology · 2020Review
- Panel 4: Recent advances in understanding the natural history of the otitis media microbiome and its response to environmental pressures.International journal of pediatric otorhinolaryngology · 2020Review
- Subversion of host immune responses by otopathogens during otitis media.Journal of leukocyte biology · 2019Review
- Vitamin D and Otitis Media.Current allergy and asthma reports · 2019Review
- Transcript Analysis Reveals a Hypoxic Inflammatory Environment in Human Chronic Otitis Media With Effusion.Frontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundChronic Otitis Media (COM) is characterized by middle ear effusion (MEE) and conductive hearing loss. MEE reflect mucus hypersecretion, but global proteomic profiling of the mucosal components are limited.
objectiveThis study aimed at characterizing the proteome of MEEs from children with COM with the goal of elucidating important innate immune responses.
methodMEEs were collected from children (n = 49) with COM undergoing myringotomy. Mass spectrometry was employed for proteomic profiling in nine samples. Independent samples were further analyzed by cytokine multiplex assay, immunoblotting, neutrophil elastase activity, next generation DNA sequencing, and/or immunofluorescence analysis.
results109 unique and common proteins were identified by MS. A majority were innate immune molecules, along with typically intracellular proteins such as histones and actin. 19.5% percent of all mapped peptide counts were from proteins known to be released by neutrophils. Immunofluorescence and immunoblotting demonstrated the presence of neutrophil extracellular traps (NETs) in every MEE, along with MUC5B colocalization. DNA found in effusions revealed unfragmented DNA of human origin.
conclusionProteomic analysis of MEEs revealed a predominantly neutrophilic innate mucosal response in which MUC5B is associated with NET DNA. NETs are a primary macromolecular constituent of human COM middle ear effusions.
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