ReviewAssay and drug development technologies2016
Avoiding Fluorescence Assay Interference-The Case for Diaphorase.
Review in Assay and drug development technologies, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- A high-sensitivity strategy to screen NAD(P)H-dependent reductase activity by coupled enzyme cascade.The Journal of biological chemistry · 2026Article
- Machine Learning-Driven Data Valuation for Optimizing High-Throughput Screening Pipelines.Journal of chemical information and modeling · 2024Article
- Machine Learning Assisted Hit Prioritization for High Throughput Screening in Drug Discovery.ACS central science · 2024Article
- Determination of pharmacological inhibition of ALDH2 by ethanol clearance in mice.Toxicology and applied pharmacology · 2024Article
- A Versatile Continuous Fluorometric Enzymatic Assay for Targeting Nicotinate Phosphoribosyltransferase.Molecules (Basel, Switzerland) · 2023Article
- Development of a High-throughput Agar Colony Formation Assay to Identify Drug Candidates against Medulloblastoma.Pharmaceuticals (Basel, Switzerland) · 2020Article
- Identification of Activators of Human Fumarate Hydratase by Quantitative High-Throughput Screening.SLAS discovery : advancing life sciences R & D · 2020Article
- Recent progress on micro- and nano-robots: towardsQuantitative imaging in medicine and surgery · 2018Article
- Discovery and optimization of piperazine-1-thiourea-based human phosphoglycerate dehydrogenase inhibitors.Bioorganic & medicinal chemistry · 2018Article
- Using Microscale Thermophoresis to Characterize Hits from High-Throughput Screening: A European Lead Factory Perspective.SLAS discovery : advancing life sciences R & D · 2018Article
- Assessing inhibitors of mutant isocitrate dehydrogenase using a suite of pre-clinical discovery assays.Scientific reports · 2017Article
- Small-Molecule Screens: A Gateway to Cancer Therapeutic Agents with Case Studies of Food and Drug Administration-Approved Drugs.Pharmacological reviews · 2017Review
- Epigenetic assays for chemical biology and drug discovery.Clinical epigenetics · 2017Review
- Diaphorase Coupling Protocols for Red-Shifting Dehydrogenase Assays.Assay and drug development technologies · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescence is utilized as the output for a range of assay formats used in high-throughput screening (HTS). Interference with these assays from the compounds in libraries utilized in HTS is a well-recognized phenomenon, particularly for assays relying on UV excitation such as for direct detection of the oxidoreductase cofactors NADH or NADPH. In this study, we discuss these interference challenges and highlight the specific case of the diaphorase/resazurin system that can be coupled to enzymes utilizing NADH or NADPH. We review the utilization of this assay system in the literature and argue that the diaphorase/resazurin system is underutilized in assay development. It is the authors' hope that this Perspective and the accompanying Technical Brief in this issue will stimulate interest in a robust and sensitive coupling system to avoid assay fluorescence interference.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.