ArticlePloS one2016
Glucose and Glycogen Metabolism in Brugia malayi Is Associated with Wolbachia Symbiont Fitness.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Preclinical animal models for onchocerciasis and loiasis: A systematic review of applications in drug screening.PLoS neglected tropical diseases · 2026Pooled it
- Secreted filarial acetylcholine analogs modulate parasite reproduction and host physiology.Nature communications · 2026Article
- Differential gene expression in Chorthippus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae: Gomphocerinae) induced by Wolbachia infection.Insect science · 2025Article
- Midgut microbiota diversity and interactions shift when field Aedes aegypti mosquitoes carry the wMel strain of Wolbachia pipientis.Communications biology · 2025Article
- Article
- Real-time PCR and immunohistochemistry detection of Wolbachia in adult Dirofilaria immitis from dogs treated with doxycycline and ivermectin.Parasites & vectors · 2025Article
- Understanding the role of trehalose in interactions betweenFrontiers in cellular and infection microbiology · 2025Review
- Repurposed Drugs That Activate Autophagy in Filarial Worms Act as Effective Macrofilaricides.Pharmaceutics · 2024Article
- A Light in the Dark: Uncovering Wolbachia-Host Interactions Using Fluorescence Imaging.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Miniature spatial transcriptomics for studying parasite-endosymbiont relationships at the micro scale.Nature communications · 2023Article
- Differences in proteome perturbations caused by the Wolbachia strain wAu suggest multiple mechanisms of Wolbachia-mediated antiviral activity.Scientific reports · 2023Article
- Down-Regulation of Lipid Metabolism in the Hepatopancreas of ShrimpInternational journal of molecular sciences · 2022Article
- Molecular Docking Simulation Studies Identifies Potential Natural Product Derived-Antiwolbachial Compounds as Filaricides against Onchocerciasis.Biomedicines · 2021Article
- Association of Wolbachia with Gene Expression in Drosophila Testes.Microbial ecology · 2021Article
- Dual RNAseq analyses at soma and germline levels reveal evolutionary innovations in the elephantiasis-agent Brugia malayi, and adaptation of its Wolbachia endosymbionts.PLoS neglected tropical diseases · 2021Article
- Diminutive, degraded but dissimilar:Microbial genomics · 2020Article
- Wolbachia and Sirtuin-4 interaction is associated with alterations in host glucose metabolism and bacterial titer.PLoS pathogens · 2020Article
- Tissue Tropisms and Transstadial Transmission of aApplied and environmental microbiology · 2020Article
- Article
- The Wolbachia Symbiont: Here, There and Everywhere.Results and problems in cell differentiation · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Wolbachia are endosymbiotic bacteria found in the majority of arthropods and filarial nematodes of medical and veterinary importance. They have evolved a wide range of symbiotic associations. In filarial nematodes that cause human lymphatic filariasis (Wuchereria bancrofti, Brugia malayi) or onchocerciasis (Onchocerca volvulus), Wolbachia are important for parasite development, reproduction and survival. The symbiotic bacteria rely in part on nutrients and energy sources provided by the host. Genomic analyses suggest that the strain of Wolbachia found in B. malayi (wBm) lacks the genes for two glycolytic enzymes--6-phosphofructokinase and pyruvate kinase--and is thus potentially unable to convert glucose into pyruvate, an important substrate for energy generation. The Wolbachia surface protein, wBm00432, is complexed to six B. malayi glycolytic enzymes, including aldolase. In this study we characterized two B. malayi aldolase isozymes and found that their expression is dependent on Wolbachia fitness and number. We confirmed by immuno-transmission electron microscopy that aldolase is associated with the Wolbachia surface. RNAi experiments suggested that aldolase-2 plays a significant role in both Wolbachia survival and embryogenesis in B. malayi. Treatment with doxycycline reduced Wolbachia fitness and increased the amount of both glucose and glycogen detected in the filarial parasite, indicating that glucose metabolism and glycogen storage in B. malayi are associated with Wolbachia fitness. This metabolic co-dependency between Wolbachia and its filarial nematode indicates that glycolysis could be a shared metabolic pathway between the bacteria and B. malayi, and thus a potential new target for anti-filarial therapy.
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