ArticleProceedings of the National Academy of Sciences of the United States of America2016
The type III secretion system apparatus determines the intracellular niche of bacterial pathogens.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 126 citations in OpenAlex.
- Profiling Selectivity for theBiochemistry · 2026Article
- A type III secretion system is required for Bordetella atropi invasion of host cells in vivo.PLoS pathogens · 2026Article
- Dual NLRC4 and non-canonical inflammasome signaling drives human GSDMD-mediated killing ofbioRxiv : the preprint server for biology · 2026Article
- IntracellularProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Escherichia coli Type III Secretion System 2 Is Associated With Patient Mortality in Bloodstream Infections.The Journal of infectious diseases · 2025Article
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- Determinants of divergentmBio · 2025Article
- The bacterial type three secretion system induces mechanoporation of vacuolar membranes.PLoS biology · 2025Article
- Pathogenic rickettsiae encode a secreted lipase that facilitates intracytosolic colonization in host cells.PloS one · 2025Article
- TheVirulence · 2024Article
- Pathogenic diversification of the gut commensalInfection and immunity · 2024Article
- Pushing boundaries: mechanisms enabling bacterial pathogens to spread between cells.Infection and immunity · 2024Review
- Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity.Cell host & microbe · 2024Article
- Evidence for intracellularJournal of bacteriology · 2024Review
- Regulation of virulence inFrontiers in microbiology · 2024Review
- TECPR1 conjugates LC3 to damaged endomembranes upon detection of sphingomyelin exposure.The EMBO journal · 2023Article
- Replicative Acinetobacter baumannii strains interfere with phagosomal maturation by modulating the vacuolar pH.PLoS pathogens · 2023Article
- Untangling Cellular Host-Pathogen Encounters at Infection Bottlenecks.Infection and immunity · 2023Review
- Engineered Escherichia coli for the in situ secretion of therapeutic nanobodies in the gut.Cell host & microbe · 2023Article
- Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Upon entry into host cells, intracellular bacterial pathogens establish a variety of replicative niches. Although some remodel phagosomes, others rapidly escape into the cytosol of infected cells. Little is currently known regarding how professional intracytoplasmic pathogens, including Shigella, mediate phagosomal escape. Shigella, like many other Gram-negative bacterial pathogens, uses a type III secretion system to deliver multiple proteins, referred to as effectors, into host cells. Here, using an innovative reductionist-based approach, we demonstrate that the introduction of a functional Shigella type III secretion system, but none of its effectors, into a laboratory strain of Escherichia coli is sufficient to promote the efficient vacuole lysis and escape of the modified bacteria into the cytosol of epithelial cells. This establishes for the first time, to our knowledge, a direct physiologic role for the Shigella type III secretion apparatus (T3SA) in mediating phagosomal escape. Furthermore, although protein components of the T3SA share a moderate degree of structural and functional conservation across bacterial species, we show that vacuole lysis is not a common feature of T3SA, as an effectorless strain of Yersinia remains confined to phagosomes. Additionally, by exploiting the functional interchangeability of the translocator components of the T3SA of Shigella, Salmonella, and Chromobacterium, we demonstrate that a single protein component of the T3SA translocon-Shigella IpaC, Salmonella SipC, or Chromobacterium CipC-determines the fate of intracellular pathogens within both epithelial cells and macrophages. Thus, these findings have identified a likely paradigm by which the replicative niche of many intracellular bacterial pathogens is established.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.