ArticlePloS one2016
Optimal Contrast Agent Staining of Ligaments and Tendons for X-Ray Computed Tomography.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Novel imaging techniques to study postmortem human fetal anatomy: a systematic review on microfocus-CT and ultra-high-field MRI.European radiology · 2020Pooled it
- 3D anatomical atlas of the heads of male and female adult Chamaeleo calyptratus.Anatomical record (Hoboken, N.J. : 2007) · 2026Article
- Synchrotron radiation micro-computed tomography of the small-spotted catshark embryonic development (Chondrichthyes: Scyliorhinus canicula).GigaScience · 2026Article
- High-Resolution 3-Dimensional Micro-CT Imaging of Intervertebral Discs Using a Novel Contrast Agent.JOR spine · 2025Article
- Evaluating the impact of contrast agents on micro and nano mechanics of soft-to-hard tissue interface.Scientific reports · 2025Article
- 2D and 3D microstructural analysis of the iliotibial tract.Journal of anatomy · 2024Article
- Understanding the Structure-Function Relationship through 3D Imaging and Biomechanical Analysis: A Novel Methodological Approach Applied to Anterior Cruciate Ligaments.Biomimetics (Basel, Switzerland) · 2024Article
- Article
- Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation.Bioengineering (Basel, Switzerland) · 2024Article
- Optimal Agents for Visualizing Collagen Tissue Microarchitecture Using Contrast-Enhanced MicroCT.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Revealing the three-dimensional murine brain microstructure by contrast-enhanced computed tomography.Frontiers in neuroscience · 2023Article
- Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues.Nature communications · 2022Article
- Bayesian inference on a microstructural, hyperelastic model of tendon deformation.Journal of the Royal Society, Interface · 2022Article
- Significance of the broad non-bony attachments of the anterior cruciate ligament on the tibial side.Scientific reports · 2022Article
- A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography.Scientific reports · 2021Article
- Three-dimensional tonotopic mapping of the human cochlea based on synchrotron radiation phase-contrast imaging.Scientific reports · 2021Article
- Exploratory Full-Field Mechanical Analysis across the Osteochondral Tissue-Biomaterial Interface in an Ovine Model.Materials (Basel, Switzerland) · 2020Article
- Phosphotungstic acid-enhanced microCT: Optimized protocols for embryonic and early postnatal mice.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography.Biological procedures online · 2020Article
- X-ray Micro-Computed Tomography for Nondestructive Three-Dimensional (3D) X-ray Histology.The American journal of pathology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
X-ray computed tomography has become an important tool for studying the microstructures of biological soft tissues, such as ligaments and tendons. Due to the low X-ray attenuation of such tissues, chemical contrast agents are often necessary to enhance contrast during scanning. In this article, the effects of using three different contrast agents--iodine potassium iodide solution, phosphotungstic acid and phosphomolybdic acid--are evaluated and compared. Porcine anterior cruciate ligaments, patellar tendons, medial collateral ligaments and lateral collateral ligaments were used as the basis of the study. Three samples of each of the four ligament/tendon types were each assigned a different contrast agent (giving a total of twelve samples), and the progression of that agent through the tissue was monitored by performing a scan every day for a total period of five days (giving a total of sixty scans). Since the samples were unstained on day one, they had been stained for a total of four days by the time of the final scans. The relative contrast enhancement and tissue deformation were measured. It was observed that the iodine potassium iodide solution penetrated the samples fastest and caused the least sample shrinkage on average (although significant deformation was observed by the time of the final scans), whereas the phosphomolybdic acid caused the greatest sample shrinkage. Equations describing the observed behaviour of the contrast agents, which can be used to predict optimal staining times for ligament and tendon X-ray computed tomography, are presented.
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