ArticlePloS one2016
Identification of Differentially Expressed Genes Associated with Litter Size in Berkshire Pig Placenta.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 34 citations in OpenAlex.
- Integrative genomic analysis reveals shared loci for reproduction and production traits in Yorkshire pigs.BMC genomics · 2025Article
- Dynamic changes in the transcriptome and metabolome of pig ovaries across developmental stages and gestation.BMC genomics · 2024Article
- Combining Transcriptomics and Proteomics to Screen Candidate Genes Related to Bovine Birth Weight.Animals : an open access journal from MDPI · 2024Article
- Identification of miR-192 target genes in porcine endometrial epithelial cells based on miRNA pull-down.Molecular biology reports · 2023Article
- Identification of long non-coding RNA-mRNA interactions and genome-wide lncRNA annotation in animal transcriptome profiling.Journal of animal science and technology · 2023Review
- Placental structure, function, and mitochondrial phenotype relate to fetal size in each fetal sex in mice†.Biology of reproduction · 2022Article
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- RNA-seq reveals downregulated osteochondral genes potentially related to tibia bacterial chondronecrosis with osteomyelitis in broilers.BMC genetics · 2020Article
- Serial gene co-expression network approach to mine biological meanings from integrated transcriptomes of the porcine endometrium during estrous cycle.Functional & integrative genomics · 2020Article
- NNature communications · 2019Article
- Hypo-phosphorylated CD147 promotes migration and invasion of hepatocellular carcinoma cells and predicts a poor prognosis.Cellular oncology (Dordrecht, Netherlands) · 2019Article
- Predicting prognosis of endometrioid endometrial adenocarcinoma on the basis of gene expression and clinical features using Random Forest.Oncology letters · 2019Article
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- Intra-tumor heterogeneity for endometrial cancer and its clinical significance.Chinese medical journal · 2019Article
- Analysis of differential gene expression of the transgenic pig with overexpression of PGC1α in muscle.Molecular biology reports · 2019Article
- Characteristics of Pig Carcass and Primal Cuts Measured by the Autofom Ⅲ Depend on Seasonal Classification.Food science of animal resources · 2019Article
- The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation.BMC genomics · 2019Article
- Factors contributing to the variation in placental efficiency on days 70, 90, and 110 of gestation in gilts.Journal of animal science · 2019Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improvement in litter size has become of great interest in the pig industry because fecundity is directly related to sow reproductive life. Improved reproduction has thus been achieved by elucidating the molecular functions of genes associated with fecundity. In the present study, we identified differentially expressed genes (DEGs) via transcriptomic analysis using RNA-sequencing (RNA-Seq) in Berkshire pig placentas from larger (LLG, mean litter size >12) and smaller (SLG, mean litter size < 6.5) litter size groups. In total 588 DEGs were identified (p < 0.05, > 1.5-fold change), of which 98 were upregulated, while 490 were downregulated in the LLG compared with the SLG. Gene Ontology (GO) enrichment was also performed. We concluded that 129 of the 588 DEGs were closely related to litter size according to reproduction related genes selected based on previous reports, as 110 genes were downregulated and 19 upregulated in the LLG compared with the SLG. RT-qPCR utilizing specific primers targeting the early growth response 2 (EGR2), pheromaxein c subunit (PHEROC) and endothelial lipase (LIPG) genes showed high accordance with RNA-Seq results. Furthermore, we investigated the upstream regulators of these three genes in the placenta. We found that WNT9B, a Wnt signaling pathway molecule, and IL-6, known inducers of EGR2 and LIPG, respectively, were significantly increased in LLG compared with SLG. We believe that the induction of IL-6 and LIPG may play an important role in increasing nutrition supply through the placenta from the sow to the piglet during gestation. These results provide novel molecular insights into pig reproduction.
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