ArticleNature protocols2016
The collection of MicroED data for macromolecular crystallography.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed, 135 citations in OpenAlex.
- Practical crystallography with a transmission electron microscope.Journal of microscopy · 2026Article
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination.IUCrJ · 2025Article
- Unraveling atomic complexity from frozen samples.Structural dynamics (Melville, N.Y.) · 2025Article
- Reaching the potential of electron diffraction.Cell reports. Physical science · 2024Article
- Structure Determination of Biogenic Crystals Directly from 3D Electron Diffraction Data.Crystal growth & design · 2024Article
- Article
- Single-crystal structure determination of nanosized metal-organic frameworks by three-dimensional electron diffraction.Nature protocols · 2022Review
- 3D electron diffraction for structure determination of small-molecule nanocrystals: A possible breakthrough for the pharmaceutical industry.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- Review
- Studying membrane proteins with MicroED.Biochemical Society transactions · 2022Article
- Electron crystallography of chiral and non-chiral small molecules.Ultramicroscopy · 2022Article
- MicroED for the study of protein-ligand interactions and the potential for drug discovery.Nature reviews. Chemistry · 2021Review
- Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED).STAR protocols · 2021Article
- MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A simple pressure-assisted method for MicroED specimen preparation.Nature communications · 2021Article
- An Overview of Microcrystal Electron Diffraction (MicroED).Annual review of biochemistry · 2021Review
- MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.Nature communications · 2021Article
- Microcrystal Electron Diffraction of Small Molecules.Journal of visualized experiments : JoVE · 2021Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The formation of large, well-ordered crystals for crystallographic experiments remains a crucial bottleneck to the structural understanding of many important biological systems. To help alleviate this problem in crystallography, we have developed the MicroED method for the collection of electron diffraction data from 3D microcrystals and nanocrystals of radiation-sensitive biological material. In this approach, liquid solutions containing protein microcrystals are deposited on carbon-coated electron microscopy grids and are vitrified by plunging them into liquid ethane. MicroED data are collected for each selected crystal using cryo-electron microscopy, in which the crystal is diffracted using very few electrons as the stage is continuously rotated. This protocol gives advice on how to identify microcrystals by light microscopy or by negative-stain electron microscopy in samples obtained from standard protein crystallization experiments. The protocol also includes information about custom-designed equipment for controlling crystal rotation and software for recording experimental parameters in diffraction image metadata. Identifying microcrystals, preparing samples and setting up the microscope for diffraction data collection take approximately half an hour for each step. Screening microcrystals for quality diffraction takes roughly an hour, and the collection of a single data set is ∼10 min in duration. Complete data sets and resulting high-resolution structures can be obtained from a single crystal or by merging data from multiple crystals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.