ArticleMethodsX2016
Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications.
Article in MethodsX, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Development of a Universal Platform for the Heterologous Expression of Bidirectional [Ni-Fe]-Hydrogenases inACS synthetic biology · 2025Article
- Redox regulation of hydrogen production in Escherichia coli during growth on by-products of the wine industry.Applied microbiology and biotechnology · 2025Article
- Improved preculture management for Cupriavidus necator cultivations.Biotechnology letters · 2023Article
- L-amino acids affect the hydrogenase activity and growth of Ralstonia eutropha H16.AMB Express · 2023Article
- Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity.Microbial cell factories · 2019Article
- Fixation of carbon dioxide by a hydrogen-oxidizing bacterium for value-added products.World journal of microbiology & biotechnology · 2018Review
- Methylacidiphilum fumariolicum SolV, a thermoacidophilic 'Knallgas' methanotroph with both an oxygen-sensitive and -insensitive hydrogenase.The ISME journal · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
The soluble hydrogenase (SH) from Ralstonia eutropha H16 is a promising candidate enzyme for H2-based biofuel application as it favours H2 oxidation and is relatively oxygen-tolerant. In this report, bioprocess development studies undertaken to produce and purify an active SH are described, based on the methods previously reported [1], [2], [3], [4]. Our modifications are: •Upstream method optimizations were undertaken on heterotrophic growth media and cell lysis involving ultrasonication.•Two anion exchangers (Q Sepharose and RESOURCE Q) and size exclusion chromatographic (Superdex 200) matrices were successfully employed for purification of a hexameric SH from R. eutropha.•The H2 oxidizing activity of the SH was demonstrated spectrophotometrically in solution and also immobilized on an EPG electrode using cyclic voltammetry.
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