ArticleBiomolecular detection and quantification2015
Effects of post-mortem and physical degradation on RNA integrity and quality.
Article in Biomolecular detection and quantification, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Viral associations in multiple sclerosis: pathogenetic mechanisms and therapeutic implications.Virology journal · 2026Review
- [Half a century of gene expression analysis : The path to modern spatial transcriptomics in autopsy tissue].Pathologie (Heidelberg, Germany) · 2026Review
- Comparative Decay Dynamics and Detectability of eDNA and eRNA in Connected and Isolated Freshwater Mesocosms Using Digital PCR.Molecular ecology resources · 2025Article
- Differential decay of multiple environmental nucleic acid components.Scientific reports · 2025Article
- Comparison Between Two Methodologies of Sample Preservation for RNA Extraction in Naturally Delivered Ovine Placenta.Animals : an open access journal from MDPI · 2025Article
- Spatial Gene Expression of Human Coronary Arteries Revealed the Molecular Features of Diffuse Intimal Thickening in Explanted Hearts.International journal of molecular sciences · 2025Article
- Detection of Gram-positive and Gram-negative bacteria in brain abscesses by 16S rRNA in situ hybridization.Journal of neuropathology and experimental neurology · 2025Article
- Integrity assay for messenger RNA in mouse and human brain samples and synaptosomal preparations.iScience · 2024Article
- Simple protocol for combined extraction of exocrine secretions and RNA in small arthropods.Biology methods & protocols · 2024Article
- Methodological considerations for aqueous environmental RNA collection, preservation, and extraction.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Utilizing the state of environmental DNA (eDNA) to incorporate time-scale information into eDNA analysis.Proceedings. Biological sciences · 2023Review
- Lessons for Understanding Central Nervous System HIV Reservoirs from the Last Gift Program.Current HIV/AIDS reports · 2022Review
- Challenges and considerations for single-cell and spatially resolved transcriptomics sample collection during spaceflight.Cell reports methods · 2022Review
- Comparative environmental RNA and DNA metabarcoding analysis of river algae and arthropods for ecological surveys and water quality assessment.Scientific reports · 2022Article
- RNA allows identifying the consumption of carrion prey.Molecular ecology resources · 2022Article
- The Effect of Tropical Temperatures on the Quality of RNA Extracted from Stabilized Whole-Blood Samples.International journal of molecular sciences · 2022Article
- Characterization of Exosomes and Exosomal RNAs Isolated from Post-Mortem Body Fluids for Molecular Forensic Diagnosis.Diagnostics (Basel, Switzerland) · 2022Article
- An investigation into simplifying total RNA extraction with minimal equipment using a low volume, electrokinetically driven microfluidic protocol.Biomicrofluidics · 2022Article
- Revisiting Rustrela Virus: New Cases of Encephalitis and a Solution to the Capsid Enigma.Microbiology spectrum · 2022Article
- Dissecting the transcriptome in cardiovascular disease.Cardiovascular research · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precision and reliability of quantitative nucleic acid analysis depends on the quality of the sample analyzed and the integrity of the nucleic acids. The integrity of RNA is currently primarily assessed by the analysis of ribosomal RNA, which is the by far dominant species. The extrapolation of these results to mRNAs and microRNAs, which are structurally quite different, is questionable. Here we show that ribosomal and some nucleolar and mitochondrial RNAs, are highly resistant to naturally occurring post-mortem degradation, while mRNAs, although showing substantial internal variability, are generally much more prone to nucleolytic degradation. In contrast, all types of RNA show the same sensitivity to heat. Using qPCR assays targeting different regions of mRNA molecules, we find no support for 5' or 3' preferentiality upon post-mortem degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.