ArticleBiomolecular detection and quantification2015
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.
Article in Biomolecular detection and quantification, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 261 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
261 citing papers in PubMed.
- A pseudo-phased genome assembly for Hemileia vastatrix reveals an isolate-specific chromosomal haploid trisomy.G3 (Bethesda, Md.) · 2026Article
- Enhanced HCN-Dependent Regulation of Temporoammonic Integration and Entorhinal-Hippocampal Epileptiform Coupling Following Febrile Seizures.Neurochemical research · 2026Article
- GABAB-Dependent Negative Feedback Contributes to Earlier Epileptiform Discharge Termination Within Malformed Cortex.International journal of molecular sciences · 2026Article
- Amino Acid Depletion Reveals Strain- and Nitrogen Source-Dependent Physiological Responses inJournal of fungi (Basel, Switzerland) · 2026Article
- Direct quantitative PCR detects genetic biomarkers of antileishmanial drug resistance in clinical samples from dogs with leishmaniosis.BMC veterinary research · 2026Article
- Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells.International journal of cardiology. Heart & vasculature · 2026Article
- Two distinct modes of Vgll4-mediated Tead regulation control organ size in zebrafish.Communications biology · 2026Article
- Understanding the qPCR Standard Curve: From Assay Validation to Absolute Quantification and Variance PCR.International journal of molecular sciences · 2026Review
- Amplification Efficiency of Quantitative PCR Reactions is Improved by Addition of Non-Target DNA.Microbial ecology · 2026Article
- Bone from Healthy Individuals and Patients with CKD Expresses the Sodium-Glucose Co-transporter-2 (SGLT2).Calcified tissue international · 2026Article
- Reporting quality of quantitative polymerase chain reaction (qPCR) methods in scientific publications.Research integrity and peer review · 2026Article
- Enhanced Early Detection and Precision Monitoring of Rubber Tree Powdery Mildew PathogenJournal of fungi (Basel, Switzerland) · 2026Article
- Subtype-Dependent Expression Patterns of Core Hippo Pathway Components in Thymic Epithelial Tumors (TETs): An RT-qPCR Study.Biomedicines · 2026Article
- Development and preliminary evaluation of real-time PCR assays for six lactic acid bacteria.Scientific reports · 2026Article
- An Insight into the Emerging Role of Extracellular Vesicle-derived Noncoding RNAs in Colorectal Cancer.MicroRNA (Shariqah, United Arab Emirates) · 2026Review
- A biomimetic and xeno-free platform for corneal engineering: synergy between PRGF technology and human dental pulp stem cells.Frontiers in bioengineering and biotechnology · 2026Article
- Development and multi-platform validation of a pan-genomic-driven PMA-qPCR method for the precise quantification of viableFrontiers in microbiology · 2026Article
- RPL28 mediates sorafenib resistance in hepatocellular carcinoma by downregulating CDC6 expression.Frontiers in oncology · 2026Article
- Fluorescent Probes for the Quantification of RNA by Real-Time qPCR.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Insights ofInternational journal of microbiology · 2026Article
201 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of technical replicates performed and the effect of the volume transferred throughout the dilution series. We used six different qPCR instruments, we performed 1-16 qPCR replicates per concentration and we tested 2-10 μl volume of analyte transferred, respectively. We find that the estimated PCR efficiency varies significantly across different instruments. Using a Monte Carlo approach, we find the uncertainty in the PCR efficiency estimation may be as large as 42.5% (95% CI) if standard curve with only one qPCR replicate is used in 16 different plates. Based on our investigation we propose recommendations for the precise estimation of PCR efficiency: (1) one robust standard curve with at least 3-4 qPCR replicates at each concentration shall be generated, (2) the efficiency is instrument dependent, but reproducibly stable on one platform, and (3) using a larger volume when constructing serial dilution series reduces sampling error and enables calibration across a wider dynamic range.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.