Evidence map›Paper›PMID 27076315›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Production of Well-Characterized Virus-like Particles in an Escherichia coli-Based Expression Platform for Preclinical Vaccine Assessments.

Newton Wahome, Anne Cooper, Prem Thapa, Shyamal Choudhari, Fei P Gao, David B Volkin, C Russell Middaugh

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Highly stableFrontiers in bioengineering and biotechnology · 2024
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Newton WahomeDepartment of Pharmaceutical Chemistry, Macromolecule and Vaccine Stabilization Center, University of Kansas, 2095 Constant Ave, Lawrence, KS, 66047, USA. wahome@ku.edu.
Anne CooperProtein Production Group, University of Kansas, Lawrence, KS, 66047, USA.
Prem ThapaMicroscopy and Analytical Imaging Lab, University of Kansas, Lawrence, KS, 66047, USA.
Shyamal ChoudhariDepartment of Pharmaceutical Chemistry, Macromolecule and Vaccine Stabilization Center, University of Kansas, 2095 Constant Ave, Lawrence, KS, 66047, USA.
Fei P GaoProtein Production Group, University of Kansas, Lawrence, KS, 66047, USA.
David B VolkinDepartment of Pharmaceutical Chemistry, Macromolecule and Vaccine Stabilization Center, University of Kansas, 2095 Constant Ave, Lawrence, KS, 66047, USA.
C Russell MiddaughDepartment of Pharmaceutical Chemistry, Macromolecule and Vaccine Stabilization Center, University of Kansas, 2095 Constant Ave, Lawrence, KS, 66047, USA.

Funding

Protein Structure Laboratory P30GM110761 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI HANZLIK, ROBERT P · 2014 to 2018
$5.6M
NIGMS NIH HHS P30GM110761
6 · The paper itself

Abstract

In this chapter we demonstrate a method to produce virus-like particles (VLPs) from Escherichia coli. Standard bacterial protocols are used for the cloning, transformation, and expression of the protein subunits. A two-step protein purification method is highlighted: one step based on separating soluble proteins with ion-exchange affinity chromatography and a second polishing step using size-exclusion columns to isolate VLP species. The ensuing VLPs can be characterized with a variety of biophysical techniques including ultraviolet (UV)-visible spectroscopy for protein quantification, dynamic light scattering for size distribution determination, and transmission electron microscopy to ascertain size and morphology.

Indexed as

Capsid ProteinsCloning, MolecularDrug Evaluation, PreclinicalDynamic Light ScatteringEscherichia coliGenetic EngineeringMicroscopy, Electron, TransmissionSpectrophotometry, UltravioletTransformation, GeneticVaccines, Virus-Like ParticleCapsid ProteinsVaccines, Virus-Like ParticleDynamic light scatteringExpressionPurificationTransmission electron microscopyUltraviolet (UV)–visible absorbance spectroscopyVirus-like particles

Identifiers

PMID27076315

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.