Evidence map›Paper›PMID 27075392›Full record

ArticleNature communications2016

Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms.

Lynne Turnbull, Masanori Toyofuku, Amelia L Hynen, Masaharu Kurosawa, Gabriella Pessi, Nicola K Petty, Sarah R Osvath, Gerardo Cárcamo-Oyarce, Erin S Gloag, Raz Shimoni and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 421 papers.

0numbers the graph read from it
0cells of the map it votes in
421citing papers in PubMed
36.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

421 citing papers in PubMed, 676 citations in OpenAlex.

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  10. DNA damage-associated vesicle production inJournal of bacteriology · 2026
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361 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors at 5 institutions in 3 countries.

Lynne TurnbullThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Masanori ToyofukuDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Amelia L HynenThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Masaharu KurosawaDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Gabriella PessiDepartment of Plant and Microbial Biology, University of Zurich, Zürich 8008, Switzerland.
Nicola K PettyThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Sarah R OsvathThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Gerardo Cárcamo-OyarceDepartment of Plant and Microbial Biology, University of Zurich, Zürich 8008, Switzerland.
Erin S GloagThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Raz ShimoniThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Ulrich OmasitsDepartment of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zürich 8093, Switzerland.
Satoshi ItoDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Xinhui YapThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Leigh G MonahanThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Rosalia CavaliereThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Christian H AhrensAgroscope, Institute for Plant Production Sciences, Research Group Molecular Diagnostics, Genomics and Bioinformatics, &Swiss Institute of Bioinformatics (SIB), Wädenswil 8820, Switzerland.
Ian G CharlesThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
Nobuhiko NomuraDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Leo EberlDepartment of Plant and Microbial Biology, University of Zurich, Zürich 8008, Switzerland.
Cynthia B WhitchurchThe ithree institute, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
University of Technology Sydney · AUUniversity of Tsukuba · JPUniversity of Zurich · CHETH Zurich · CHSIB Swiss Institute of Bioinformatics · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many bacteria produce extracellular and surface-associated components such as membrane vesicles (MVs), extracellular DNA and moonlighting cytosolic proteins for which the biogenesis and export pathways are not fully understood. Here we show that the explosive cell lysis of a sub-population of cells accounts for the liberation of cytosolic content in Pseudomonas aeruginosa biofilms. Super-resolution microscopy reveals that explosive cell lysis also produces shattered membrane fragments that rapidly form MVs. A prophage endolysin encoded within the R- and F-pyocin gene cluster is essential for explosive cell lysis. Endolysin-deficient mutants are defective in MV production and biofilm development, consistent with a crucial role in the biogenesis of MVs and liberation of extracellular DNA and other biofilm matrix components. Our findings reveal that explosive cell lysis, mediated through the activity of a cryptic prophage endolysin, acts as a mechanism for the production of bacterial MVs.

Indexed as

BacteriolysisBiofilmsOrganelle BiogenesisCell MembraneDNA, BacterialEndopeptidasesExtracellular SpacePseudomonas aeruginosaPyocinsQuinolonesStress, Physiological2-heptyl-3-hydroxy-4-quinoloneDNA, BacterialendolysinEndopeptidasesPyocinsQuinolones

Identifiers

PMID27075392
PMCPMC4834629
OpenAlexW2335807995

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.