ArticleMolecular biology of the cell2016
The F-actin bundler α-actinin Ain1 is tailored for ring assembly and constriction during cytokinesis in fission yeast.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Curving Actin Across Systems: Do Axonal Actin Rings Share Common Underlying Mechanisms?Journal of neurochemistry · 2026Review
- Fast Actin Disassembly and Fimbrin Mechanosensitivity Support Rapid Turnover in a Model of Clathrin-Mediated Endocytosis.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Mechanical and biochemical feedback combine to generate complex contractile oscillations in cytokinesis.Current biology : CB · 2024Article
- Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.Frontiers in cell and developmental biology · 2024Article
- Actin turnover protects the cytokinetic contractile ring from structural instability.Journal of cell science · 2023Article
- Review
- Formin Cdc12's specific actin assembly properties are tailored for cytokinesis in fission yeast.Biophysical journal · 2021Article
- Loss of Septation Initiation Network (SIN) kinases blocks tissue invasion and unlocks echinocandin cidal activity against Aspergillus fumigatus.PLoS pathogens · 2021Article
- Actin bundle architecture and mechanics regulate myosin II force generation.Biophysical journal · 2021Article
- 3D-printed gelatin methacrylate (GelMA)/silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration.Regenerative biomaterials · 2021Article
- F-Actin Cytoskeleton Network Self-Organization Through Competition and Cooperation.Annual review of cell and developmental biology · 2020Review
- Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast.Molecular biology of the cell · 2020Article
- Bond Type and Discretization of Nonmuscle Myosin II Are Critical for Simulated Contractile Dynamics.Biophysical journal · 2020Article
- Article
- Article
- Mechanical and kinetic factors drive sorting of F-actin cross-linkers on bundles.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular form and function of the cytokinetic ring.Journal of cell science · 2019Review
- Article
- Empowering statistical methods for cellular and molecular biologists.Molecular biology of the cell · 2019Article
- Network Contractility During Cytokinesis-from Molecular to Global Views.Biomolecules · 2019Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The actomyosin contractile ring is a network of cross-linked actin filaments that facilitates cytokinesis in dividing cells. Contractile ring formation has been well characterized in Schizosaccharomyces pombe, in which the cross-linking protein α-actinin SpAin1 bundles the actin filament network. However, the specific biochemical properties of SpAin1 and whether they are tailored for cytokinesis are not known. Therefore we purified SpAin1 and quantified its ability to dynamically bind and bundle actin filaments in vitro using a combination of bulk sedimentation assays and direct visualization by two-color total internal reflection fluorescence microscopy. We found that, while SpAin1 bundles actin filaments of mixed polarity like other α-actinins, SpAin1 has lower bundling activity and is more dynamic than human α-actinin HsACTN4. To determine whether dynamic bundling is important for cytokinesis in fission yeast, we created the less dynamic bundling mutant SpAin1(R216E). We found that dynamic bundling is critical for cytokinesis, as cells expressing SpAin1(R216E) display disorganized ring material and delays in both ring formation and constriction. Furthermore, computer simulations of initial actin filament elongation and alignment revealed that an intermediate level of cross-linking best facilitates filament alignment. Together our results demonstrate that dynamic bundling by SpAin1 is important for proper contractile ring formation and constriction.
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