ArticleBMC biology2016
Muscle cell identity requires Pax7-mediated lineage-specific DNA demethylation.
Article in BMC biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Machine learning-driven classification and prognostic prediction of kidney renal clear cell carcinoma using APOBEC family expression signatures.Scientific reports · 2025Article
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- Integrative genetic and epigenetic control of skeletal muscle fiber traits in agricultural animals.Frontiers in genetics · 2025Review
- Exploring the reciprocity between pioneer factors and development.Development (Cambridge, England) · 2024Review
- APOBEC2 safeguards skeletal muscle cell fate through binding chromatin and regulating transcription of non-muscle genes during myoblast differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Skeletal muscle feature of different populations in large yellow croaker (Frontiers in molecular biosciences · 2024Article
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- Article
- The Art of Reprogramming for Regenerative Medicine.Frontiers in cell and developmental biology · 2022Review
- Article
- Pathomechanisms and biomarkers in facioscapulohumeral muscular dystrophy: roles of DUX4 and PAX7.EMBO molecular medicine · 2021Review
- A comprehensive epigenome atlas reveals DNA methylation regulating skeletal muscle development.Nucleic acids research · 2021Article
- The Gentle Side of the UPS: Ubiquitin-Proteasome System and the Regulation of the Myogenic Program.Frontiers in cell and developmental biology · 2021Review
- Transcriptome and epigenome diversity and plasticity of muscle stem cells following transplantation.PLoS genetics · 2020Article
- Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation.Epigenetics & chromatin · 2019Article
- Master control: transcriptional regulation of mammalian Myod.Journal of muscle research and cell motility · 2019Review
- PAX7 target genes are globally repressed in facioscapulohumeral muscular dystrophy skeletal muscle.Nature communications · 2017Article
- Myogenic progenitor specification from pluripotent stem cells.Seminars in cell & developmental biology · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundSkeletal muscle stem cells enable the formation, growth, maintenance, and regeneration of skeletal muscle throughout life. The regeneration process is compromised in several pathological conditions, and muscle progenitors derived from pluripotent stem cells have been suggested as a potential therapeutic source for tissue replacement. DNA methylation is an important epigenetic mechanism in the setting and maintenance of cellular identity, but its role in stem cell determination towards the myogenic lineage is unknown. Here we addressed the DNA methylation dynamics of the major genes orchestrating the myogenic determination and differentiation programs in embryonic stem (ES) cells, their Pax7-induced myogenic derivatives, and muscle stem cells in proliferating and differentiating conditions.
resultsOur data showed a common muscle-specific DNA demethylation signature required to acquire and maintain the muscle-cell identity. This specific-DNA demethylation is Pax7-mediated, and it is a prime event in muscle stem cells gene activation. Notably, downregulation of the demethylation-related enzyme Apobec2 in ES-derived myogenic precursors reduced myogenin-associated DNA demethylation and dramatically impaired the expression of differentiation markers and, ultimately, muscle differentiation.
conclusionsOur results underscore DNA demethylation as a key mechanism driving myogenesis and identify specific Pax7-mediated DNA demethylation signatures to acquire and maintain the muscle-cell identity. Additionally, we provide a panel of epigenetic markers for the efficient and safe generation of ES- and induced pluripotent stem cell (iPS)-derived myogenic progenitors for therapeutic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.