Evidence map›Paper›PMID 27075036›Full record

ArticleCerebral cortex (New York, N.Y. : 1991)2017

Homer1 Scaffold Proteins Govern Ca2+ Dynamics in Normal and Reactive Astrocytes.

Lara Buscemi, Vanessa Ginet, Jan Lopatar, Vedrana Montana, Luca Pucci, Paola Spagnuolo, Tamara Zehnder, Vladimir Grubišic, Anita Truttman, Carlo Sala and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cerebral cortex (New York, N.Y. : 1991), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 47 citations in OpenAlex.

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  16. Exocytosis in Astrocytes.Biomolecules · 2021
    Review
  17. Article
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  19. Neurotransmitter system aberrations in patients with drug addiction.Journal of neural transmission (Vienna, Austria : 1996) · 2020
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 4 countries.

Lara BuscemiDepartment of Fundamental Neurosciences, University of Lausanne, CH1005 Lausanne, Switzerland.
Vanessa GinetDepartment of Fundamental Neurosciences, University of Lausanne, CH1005 Lausanne, Switzerland.
Jan LopatarDepartment of Fundamental Neurosciences, University of Lausanne, CH1005Lausanne, Switzerland.
Vedrana MontanaDepartment of Biotechnology, University of Rijeka, 51000 Rijeka, Croatia.
Luca PucciDepartment of Fundamental Neurosciences, University of Lausanne, CH1005Lausanne, Switzerland.
Paola SpagnuoloDepartment of Fundamental Neurosciences, University of Lausanne, CH1005 Lausanne, Switzerland.
Tamara ZehnderDepartment of Fundamental Neurosciences, University of Lausanne, CH1005Lausanne, Switzerland.
Vladimir GrubišicDepartment of Neurobiology, Center for Glial Biology in Medicine, Civitan International Research Center, Atomic Force Microscopy and Nanotechnology Laboratories, and Evelyn F. McKnight Brain Institute, University of Alabama at Birmingham, Birmingham, AL, USA.
Anita TruttmanDivision of Neonatology, Department of Paediatrics and Paediatric Surgery, University Hospital Centre and University of Lausanne, CH-1015 Lausanne, Switzerland.
Carlo SalaCNR Institute of Neuroscience and Department of Biotechnology and Translational Medicine, University of Milan, Milan, Italy.
Lorenz HirtStroke Laboratory, Neurology Service, Department of Clinical Neurosciences, University Hospital Centre and University of Lausanne, CH-1011 Lausanne, Switzerland.
Vladimir ParpuraDepartment of Neurobiology, Center for Glial Biology in Medicine, Civitan International Research Center, Atomic Force Microscopy and Nanotechnology Laboratories, and Evelyn F. McKnight Brain Institute, University of Alabama at Birmingham, Birmingham, AL, USA.
Julien PuyalDepartment of Fundamental Neurosciences, University of Lausanne, CH1005 Lausanne, Switzerland.
Paola BezziDepartment of Fundamental Neurosciences, University of Lausanne, CH1005Lausanne, Switzerland.
University of Lausanne · CHUniversity of Alabama at Birmingham · USCivitan International · USUniversity of Milan · IT

Funding

The Role of Astroglia in the Enteric Nervous System and Gut FunctionR21HD078678 · NICHD · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI PARPURA, VLADIMIR · 2014 to 2015
$400k
NICHD NIH HHS R21 HD078678
6 · The paper itself

Abstract

In astrocytes, the intracellular calcium (Ca2+) signaling mediated by activation of metabotropic glutamate receptor 5 (mGlu5) is crucially involved in the modulation of many aspects of brain physiology, including gliotransmission. Here, we find that the mGlu5-mediated Ca2+ signaling leading to release of glutamate is governed by mGlu5 interaction with Homer1 scaffolding proteins. We show that the long splice variants Homer1b/c are expressed in astrocytic processes, where they cluster with mGlu5 at sites displaying intense local Ca2+ activity. We show that the structural and functional significance of the Homer1b/c-mGlu5 interaction is to relocate endoplasmic reticulum (ER) to the proximity of the plasma membrane and to optimize Ca2+ signaling and glutamate release. We also show that in reactive astrocytes the short dominant-negative splice variant Homer1a is upregulated. Homer1a, by precluding the mGlu5-ER interaction decreases the intensity of Ca2+ signaling thus limiting the intensity and the duration of glutamate release by astrocytes. Hindering upregulation of Homer1a with a local injection of short interfering RNA in vivo restores mGlu5-mediated Ca2+ signaling and glutamate release and sensitizes astrocytes to apoptosis. We propose that Homer1a may represent one of the cellular mechanisms by which inflammatory astrocytic reactions are beneficial for limiting brain injury.

Indexed as

AnimalsAstrocytesBrain IschemiaCalciumCations, DivalentCells, CulturedCerebral CortexEndoplasmic ReticulumGlial Fibrillary Acidic ProteinGlutamic AcidGreen Fluorescent ProteinsHomer Scaffolding ProteinsHumansInfant, NewbornMaleMice, TransgenicCalciumCations, Divalentenhanced green fluorescent proteinGlial Fibrillary Acidic ProteinGlutamic AcidGreen Fluorescent ProteinsHOMER1 protein, humanHomer1 protein, mouseHomer1 protein, ratHomer Scaffolding Proteinsmetabotropic glutamate receptor type 1Receptor, Metabotropic Glutamate 5Receptors, Metabotropic GlutamateastrocytesHomerintracellular Ca2+mGlu5neuroinflammation

Identifiers

PMID27075036
PMCPMC5963825
OpenAlexW2338195210

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.