ArticleBiophysical journal2016
Role of Inverse-Cone-Shape Lipids in Temperature-Controlled Self-Reproduction of Binary Vesicles.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Devising a divisome for synthetic cells.Nature reviews. Chemistry · 2026Review
- Growth of fatty acid vesicles coupled with amino acid sequences of peptides toward evolvable protocells.Communications chemistry · 2026Article
- Flippase-Mediated Hybrid Vesicle Division.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Protocell Dynamics: Modelling Growth and Division of Lipid Vesicles Driven by an Autocatalytic Reaction.Life (Basel, Switzerland) · 2025Article
- Concepts of a synthetic minimal cell: Information molecules, metabolic pathways, and vesicle reproduction.Biophysics and physicobiology · 2024Article
- Synthesising a minimal cell with artificial metabolic pathways.Communications chemistry · 2023Article
- Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles.Pharmaceutics · 2022Article
- Evolution of Proliferative Model Protocells Highly Responsive to the Environment.Life (Basel, Switzerland) · 2022Review
- Shape Deformation, Budding and Division of Giant Vesicles and Artificial Cells: A Review.Life (Basel, Switzerland) · 2022Review
- Identifying and Manipulating Giant Vesicles: Review of Recent Approaches.Micromachines · 2022Review
- Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.Frontiers in bioengineering and biotechnology · 2022Article
- Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference.Life (Basel, Switzerland) · 2021Article
- Liposomes: Structure, Biomedical Applications, and Stability Parameters With Emphasis on Cholesterol.Frontiers in bioengineering and biotechnology · 2021Review
- Morphologies of Vesicle Doublets: Competition among Bending Elasticity, Surface Tension, and Adhesion.Biophysical journal · 2020Article
- Ectopic Neo-Formed Intracellular Membranes inBiomolecules · 2018Review
- Investigating cell functioning by theoretical analysis of cell-to-cell variability.European biophysics journal : EBJ · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigate a temperature-driven recursive division of binary giant unilamellar vesicles (GUVs). During the heating step of the heating-cooling cycle, the spherical mother vesicle deforms to a budded limiting shape using up the excess area produced by the chain melting of the lipids and then splits off into two daughter vesicles. Upon cooling, the daughter vesicle opens a pore and recovers the spherical shape of the mother vesicle. Our GUVs are composed of DLPE (1,2-dilauroyl-sn-glycero-3-phosphoethanolamine) and DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine). During each cycle, vesicle deformation is monitored by a fast confocal microscope and the images are analyzed to obtain the time evolution of reduced volume and reduced monolayer area difference as the key geometric parameters that quantify vesicle shape. By interpreting the deformation pathway using the area-difference elasticity theory, we conclude that vesicle division relies on (1) a tiny asymmetric distribution of DLPE within the bilayer, which controls the observed deformation from the sphere to the budded shape; and (2) redistribution of DLPE during the deformation-division stage, which ensures that the process is recursive. The spontaneous coupling between membrane curvature and PE lipid distribution is responsible for the observed recursive division of GUVs. These results shed light on the mechanisms of vesicle self-reproduction.
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