ArticleBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica2016
Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts.
Article in Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Genetic polymorphisms and protein levels in vocal fold leukoplakia: a systematic review.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2022Pooled it
- Titanium Dioxide Particle Size Influences Macrophage Signal Switch Towards Osteogenesis In Vitro.International dental journal · 2026Article
- Expression of BMP-2, OPG, RANKL And Number of Osteoblasts After Application of Bovine Amniotic Membrane and Secretome Umbilical Cord in Wistar Rats.Nanotheranostics · 2026Article
- Chronic stress and the IL-10-mediated immunoregulatory loop in the pathogenesis of periodontitis.Clinical science (London, England : 1979) · 2025Review
- Effect of Mechanical Force Stress on the Inflammatory Response in Human Periodontal Ligament Cells.International dental journal · 2025Review
- sCD14-ST and Related Osteoimmunological Biomarkers: A New Diagnostic Approach to Osteomyelitis.Diagnostics (Basel, Switzerland) · 2024Article
- Characterisation and Expression of Osteogenic and Periodontal Markers of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) from Diabetic Knee Joints.International journal of molecular sciences · 2024Article
- Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts.Biomedicines · 2024Article
- Review
- GDF15 Promotes the Osteogenic Cell Fate of Periodontal Ligament Fibroblasts, thus Affecting Their Mechanobiological Response.International journal of molecular sciences · 2023Article
- Melatonin inhibits osteoclastogenesis via RANKL/OPG suppression mediated by Rev-Erbα in osteoblasts.Journal of cellular and molecular medicine · 2022Article
- Peroxisome proliferator-activated receptor gamma preserves intracellular homeostasis of insulin-resistant periodontal ligament stem cells.Annals of translational medicine · 2022Article
- Hydroxyapatite Use in Spine Surgery-Molecular and Clinical Aspect.Materials (Basel, Switzerland) · 2022Review
- The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium.Bioengineering (Basel, Switzerland) · 2021Review
- Programmable immune activating electrospun fibers for skin regeneration.Bioactive materials · 2021Article
- Role of nitric oxide in orthodontic tooth movement (Review).International journal of molecular medicine · 2021Review
- The Impact of Hyperbaric Oxygen Therapy on Serum C-Reactive Protein Levels, Osteoprotegerin Expression, and Osteoclast Numbers in Induced-Periodontitis Diabetic Rats.European journal of dentistry · 2020Article
- Loss of interleukin-10 exacerbates early Type-1 diabetes-induced bone loss.Journal of cellular physiology · 2020Article
- Diabetes Activates Periodontal Ligament Fibroblasts via NF-κB In Vivo.Journal of dental research · 2018Article
- Effects of Local Antibiotic Delivery from Porous Space Maintainers on Infection Clearance and Induction of an Osteogenic Membrane in an Infected Bone Defect.Tissue engineering. Part A · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.