ArticleBioMed research international2016
Cloning and Expression of the γ-Polyglutamic Acid Synthetase Gene pgsBCA in Bacillus subtilis WB600.
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Citrate Supplementation Modulates Medium Viscosity and Poly-γ-Glutamic Acid Synthesis by EngineeredEngineering in life sciences · 2025Article
- Recent Advances in Microbial Synthesis of Poly-γ-Glutamic Acid: A Review.Foods (Basel, Switzerland) · 2022Review
- High copy number and highly stable Escherichia coli-Bacillus subtilis shuttle plasmids based on pWB980.Microbial cell factories · 2020Article
- Identification of Key Metabolites in Poly-γ-Glutamic Acid Production by Tuning γ-PGA Synthetase Expression.Frontiers in bioengineering and biotechnology · 2020Article
- A novel unhairing enzyme produced by heterologous expression of keratinase gene (kerT) in Bacillus subtilis.World journal of microbiology & biotechnology · 2019Article
- Poly-γ-glutamic Acid Synthesis, Gene Regulation, Phylogenetic Relationships, and Role in Fermentation.International journal of molecular sciences · 2017Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To clone and express the γ-polyglutamic acid (γ-PGA) synthetase gene pgsBCA in Bacillus subtilis, a pWB980 plasmid was used to construct and transfect the recombinant expression vector pWB980-pgsBCA into Bacillus subtilis WB600. PgsBCA was expressed under the action of a P43 promoter in the pWB980 plasmid. Our results showed that the recombinant bacteria had the capacity to synthesize γ-PGA. The expression product was secreted extracellularly into the fermentation broth, with a product yield of 1.74 g/L or higher. γ-PGA samples from the fermentation broth were purified and characterized. Hydrolysates of γ-PGA presented in single form, constituting simple glutamic acid only, which matched the characteristics of the infrared spectra of the γ-PGA standard, and presented as multimolecular aggregates with a molecular weight within the range of 500-600 kDa. Expressing the γ-PGA synthetase gene pgsBCA in B. subtilis system has potential industrial applications.
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