ArticleBlood2016
Gene disruption of dematin causes precipitous loss of erythrocyte membrane stability and severe hemolytic anemia.
Article in Blood, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Severe pathological and transcriptional changes in haematopoietic organs of salmon suffering from salmon gill poxvirus disease.BMC veterinary research · 2025Article
- Mutations in linker-2 of KLF1 impair expression of membrane transporters and cytoskeletal proteins causing hemolysis.Nature communications · 2024Article
- The Role of Liquid-Liquid Phase Separation in Actin Polymerization.International journal of molecular sciences · 2023Review
- Dematin Regulates Calcium Mobilization, Thrombosis, and Early Akt Activation in Platelets.Molecular and cellular biology · 2023Article
- Partial loss-of-function variant in neuregulin 1 identified in family with heritable peripheral neuropathy.Human mutation · 2022Article
- Physiology and Proteomic Basis of Lung Adaptation to High-Altitude Hypoxia in Tibetan Sheep.Animals : an open access journal from MDPI · 2022Article
- Self-construction of actin networks through phase separation-induced abLIM1 condensates.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Proteomic and functional analyses of the periodic membrane skeleton in neurons.Nature communications · 2022Article
- F-actin-bundling sites are conserved in proteins with villin-type headpiece domains.Molecular biology of the cell · 2020Article
- Impact of genotype, body weight and sex on the prenatal muscle transcriptome of Iberian pigs.PloS one · 2020Article
- abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing.Cell discovery · 2018Article
- Proteomics of Stored Red Blood Cell Membrane and Storage-Induced Microvesicles Reveals the Association of Flotillin-2 With Band 3 Complexes.Frontiers in physiology · 2018Article
- Stabilization of F-actin by tropomyosin isoforms regulates the morphology and mechanical behavior of red blood cells.Molecular biology of the cell · 2017Article
- Tropomodulin 1 controls erythroblast enucleation via regulation of F-actin in the enucleosome.Blood · 2017Article
- Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.Scientific reports · 2017Article
- Orchestration of late events in erythropoiesis by KLF1/EKLF.Current opinion in hematology · 2017Review
- Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia.Antioxidants & redox signaling · 2017Review
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Dematin is a relatively low abundance actin binding and bundling protein associated with the spectrin-actin junctions of mature erythrocytes. Primary structure of dematin includes a loosely folded core domain and a compact headpiece domain that was originally identified in villin. Dematin's actin binding properties are regulated by phosphorylation of its headpiece domain by cyclic adenosine monophosphate-dependent protein kinase. Here, we used a novel gene disruption strategy to generate the whole body dematin gene knockout mouse model (FLKO). FLKO mice, while born at a normal Mendelian ratio, developed severe anemia and exhibited profound aberrations of erythrocyte morphology and membrane stability. Having no apparent effect on primitive erythropoiesis, FLKO mice show significant enhancement of erythroblast enucleation during definitive erythropoiesis. Using membrane protein analysis, domain mapping, electron microscopy, and dynamic deformability measurements, we investigated the mechanism of membrane instability in FLKO erythrocytes. Although many membrane and cytoskeletal proteins remained at their normal levels, the major peripheral membrane proteins spectrin, adducin, and actin were greatly reduced in FLKO erythrocytes. Our results demonstrate that dematin plays a critical role in maintaining the fundamental properties of the membrane cytoskeleton complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.