ArticleNature communications2016
In situ regeneration of bioactive coatings enabled by an evolved Staphylococcus aureus sortase A.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Biomimetic layered, ecological, advanced, multi-functional film for sustainable packaging.Nature communications · 2025Article
- Site-Specific Functionalization of Recombinant Spider Silk Using Enzymatic Sortase Coupling.ACS omega · 2025Article
- Article
- Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs.JACC. Basic to translational science · 2023Article
- Engineering Ligand and Receptor Pairs with LIPSTIC to Track Cell-Cell Interactions.Current protocols · 2021Article
- Encapsulation of murine hematopoietic stem and progenitor cells in a thiol-crosslinked maleimide-functionalized gelatin hydrogel.Acta biomaterialia · 2021Article
- A rechargeable anti-thrombotic coating for blood-contacting devices.Biomaterials · 2021Article
- Biomimetic Hydrogels to Promote Wound Healing.Frontiers in bioengineering and biotechnology · 2021Review
- Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Next-Generation Biomaterials for Culture and Manipulation of Stem Cells.Cold Spring Harbor perspectives in biology · 2020Article
- β-Hydroxy-Stabilized Boron-Nitrogen Heterocycles Enable Rapid and Efficient C-Terminal Protein Modification.Bioconjugate chemistry · 2019Article
- Genetically Encoded Photocleavable Linkers for Patterned Protein Release from Biomaterials.Journal of the American Chemical Society · 2019Article
- Broadening the scope of sortagging.RSC advances · 2019Review
- Recent progress in enzymatic protein labelling techniques and their applications.Chemical Society reviews · 2018Review
- Bioorthogonal Strategies for Engineering Extracellular Matrices.Advanced functional materials · 2018Article
- Article
- Immobilization of Staphylococcus aureus Sortase A on Chitosan Particles and Its Applications in Peptide-to-Peptide Ligation and Peptide Cyclization.Molecules (Basel, Switzerland) · 2018Article
- Recent advances in covalent, site-specific protein immobilization.F1000Research · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Surface immobilization of bioactive molecules is a central paradigm in the design of implantable devices and biosensors with improved clinical performance capabilities. However, in vivo degradation or denaturation of surface constituents often limits the long-term performance of bioactive films. Here we demonstrate the capacity to repeatedly regenerate a covalently immobilized monomolecular thin film of bioactive molecules through a two-step stripping and recharging cycle. Reversible transpeptidation by a laboratory evolved Staphylococcus aureus sortase A (eSrtA) enabled the rapid immobilization of an anti-thrombogenic film in the presence of whole blood and permitted multiple cycles of film regeneration in vitro that preserved its biological activity. Moreover, eSrtA transpeptidation facilitated surface re-engineering of medical devices in situ after in vivo implantation through removal and restoration film constituents. These studies establish a rapid, orthogonal and reversible biochemical scheme to regenerate selective molecular constituents with the potential to extend the lifetime of bioactive films.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.