ArticleThe Journal of antibiotics2016
Genome mining of the sordarin biosynthetic gene cluster from Sordaria araneosa Cain ATCC 36386: characterization of cycloaraneosene synthase and GDP-6-deoxyaltrose transferase.
Article in The Journal of antibiotics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- BioprospectingFrontiers in fungal biology · 2021Pooled it
- Uncovering a novel biosynthetic gene cluster for sordarin through genome mining in the fungus Talaromyces adpressus.Bioresources and bioprocessing · 2025Article
- Biocatalyzed and Photochemical Formal [2+2] Cycloaddition of Euphoboetirane A: A Route to a Fused Pentacyclic Diterpene Skeleton.Organic letters · 2025Article
- Substrate promiscuity catalyzed by anMycology · 2025Article
- Discovery and heterologous biosynthesis of glycosylated polyketide luteodienoside A reveals unprecedented glucinol-mediated product offloading by a fungal carnitineChemical science · 2024Article
- Bioinformatic Analysis of Secondary Metabolite Biosynthetic Potential in PathogenicJournal of fungi (Basel, Switzerland) · 2023Article
- Combating Antimicrobial Resistance in the Post-Genomic Era: Rapid Antibiotic Discovery.Molecules (Basel, Switzerland) · 2023Review
- Genome-Based Analysis ofBiology · 2022Article
- Discovery and characterization of a terpene biosynthetic pathway featuring a norbornene-forming Diels-Alderase.Nature communications · 2022Article
- High Diversity of Type I Polyketide Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- Diterpenes Specially Produced by Fungi: Structures, Biological Activities, and Biosynthesis (2010-2020).Journal of fungi (Basel, Switzerland) · 2022Review
- Characterization of a new fusicoccane-type diterpene synthase and an associated P450 enzyme.Beilstein journal of organic chemistry · 2022Article
- Synthaser: a CD-Search enabled Python toolkit for analysing domain architecture of fungal secondary metabolite megasynth(et)ases.Fungal biology and biotechnology · 2021Article
- Secondary metabolite biosynthetic diversity in the fungal familyStudies in mycology · 2021Article
- Sequencing and Analysis of the Entire Genome of the Mycoparasitic Fungus Trichoderma afroharzianum.Microbiology resource announcements · 2021Article
- Three New Derivatives of Zopfinol fromJournal of fungi (Basel, Switzerland) · 2021Article
- GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential.Frontiers in molecular biosciences · 2021Review
- Two Diterpene Synthases from Chryseobacterium: Chryseodiene Synthase and Wanjudiene Synthase.Angewandte Chemie (International ed. in English) · 2020Article
- Thioesterase-Catalyzed Aminoacylation and Thiolation of Polyketides in Fungi.Journal of the American Chemical Society · 2019Article
- In silico prediction and characterisation of secondary metabolite clusters in the plant pathogenic fungus Verticillium dahliae.FEMS microbiology letters · 2019Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sordarin is a glycoside antibiotic with a unique tetracyclic diterpene aglycone structure called sordaricin. To understand its intriguing biosynthetic pathway that may include a Diels-Alder-type [4+2]cycloaddition, genome mining of the gene cluster from the draft genome sequence of the producer strain, Sordaria araneosa Cain ATCC 36386, was carried out. A contiguous 67 kb gene cluster consisting of 20 open reading frames encoding a putative diterpene cyclase, a glycosyltransferase, a type I polyketide synthase, and six cytochrome P450 monooxygenases were identified. In vitro enzymatic analysis of the putative diterpene cyclase SdnA showed that it catalyzes the transformation of geranylgeranyl diphosphate to cycloaraneosene, a known biosynthetic intermediate of sordarin. Furthermore, a putative glycosyltransferase SdnJ was found to catalyze the glycosylation of sordaricin in the presence of GDP-6-deoxy-d-altrose to give 4'-O-demethylsordarin. These results suggest that the identified sdn gene cluster is responsible for the biosynthesis of sordarin. Based on the isolated potential biosynthetic intermediates and bioinformatics analysis, a plausible biosynthetic pathway for sordarin is proposed.
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