ArticleJournal of applied toxicology : JAT2016
MicroRNA profiles in a monkey testicular injury model induced by testicular hyperthermia.
Article in Journal of applied toxicology : JAT, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- miR-132-y TargetsBiomolecules · 2026Article
- Circulating microRNAs as promising testicular translatable safety biomarkers: current state and future perspectives.Archives of toxicology · 2023Review
- Role of microRNA-34b-5p in cancer and injury: how does it work?Cancer cell international · 2022Review
- Sertoli cell-derived exosome-mediated transfer of miR-145-5p inhibits Leydig cell steroidogenesis by targeting steroidogenic factor 1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Significance and Relevance of Spermatozoal RNAs to Male Fertility in Livestock.Frontiers in genetics · 2021Review
- A RNA-Seq Analysis to Describe the Boar Sperm Transcriptome and Its Seasonal Changes.Frontiers in genetics · 2019Article
- Routes of Zika virus dissemination in the testis and epididymis of immunodeficient mice.Nature communications · 2018Article
- Circulating liver-specific microRNAs in cynomolgus monkeys.Journal of toxicologic pathology · 2018Review
- Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection.BMC genomics · 2017Article
- MicroRNA profiles in a monkey testicular injury model induced by testicular hyperthermia.Journal of applied toxicology : JAT · 2016Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
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Abstract
To characterize microRNAs (miRNAs) involved in testicular toxicity in cynomolgus monkeys, miRNA profiles were investigated using next-generation sequencing (NGS), microarray and reverse transcription-quantitative real-time-PCR (RT-qPCR) methods. First, to identify organ-specific miRNAs, we compared the expression levels of miRNAs in the testes to those in representative organs (liver, heart, kidney, lung, spleen and small intestine) obtained from naïve mature male and female monkeys (n = 2/sex) using NGS analysis. Consequently, miR-34c-5p, miR-202-5p, miR-449a and miR-508-3p were identified to be testicular-specific miRNAs in cynomolgus monkeys. Next, we investigated miRNA profiles after testicular-hyperthermia (TH) treatment to determine which miRNAs are involved in testicular injury. In this experiment, mature male monkeys were divided into groups with or without TH-treatment (n = 3/group) by immersion of the testes in a water bath at 43 °C for 30 min for 5 consecutive days. As a result, TH treatment induced testicular injury in all animals, which was characterized by decreased numbers of spermatocytes and spermatids. In a microarray analysis of the testis, 11 up-regulated (>2.0 fold) and 13 down-regulated (<0.5 fold) miRNAs were detected compared with those in the control animals. Interestingly, down-regulated miRNAs included two testicular-specific miRNAs, miR-34c-5p and miR-449a, indicating their potential use as biomarkers for testicular toxicity. Furthermore, RT-qPCR analysis revealed decreased expression levels of testicular miR-34b-5p and miR-34c-5p, which are enriched in meiotic cells, reflecting the decrease in pachytene spermatocytes and spermatids after TH treatment. These results provide valuable insights into the mechanism of testicular toxicity and potential translational biomarkers for testicular toxicity. Copyright © 2016 The Authors. Journal of Applied Toxicology published by John Wiley & Sons Ltd.
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