ArticleCurrent aging science2016
Age and High-Fat Diet Effects on Glutamine Synthetase Immunoreactivity in Liver and Hippocampus and Recognition Memory in Mice.
Article in Current aging science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Dietary Fatty Acids Differentially Modulate Hippocampal Glutamate-Related Gene Expression in Mice: Sex-Specific in Vivo and in Vitro Effects.Molecular nutrition & food research · 2026Article
- High fat diet reveals sex-specific fecal and liver metabolic alterations in C57BL/6J obese mice.Metabolomics : Official journal of the Metabolomic Society · 2023Article
- The contribution of astrocytes to obesity-associated metabolic disturbances.Journal of biomedical research · 2022Article
- Integrative analysis of physiological responses to high fat feeding with diffusion tensor images and neurochemical profiles of the mouse brain.International journal of obesity (2005) · 2021Article
- CPS1: Looking at an ancient enzyme in a modern light.Molecular genetics and metabolism · 2020Review
- Obesity-related cognitive impairment: The role of endothelial dysfunction.Neurobiology of disease · 2019Review
- Effects of HIV-1 TAT protein and methamphetamine exposure on visual discrimination and executive function in mice.Behavioural brain research · 2018Article
- Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet.Frontiers in neuroscience · 2018Article
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8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHigh-Fat Diet (HFD)-induced obesity may promote agerelated memory impairment via disturbances of ammonia-glutamine metabolism.
objectiveWe studied the effects of age and long-term HFD exposure on Glutamine Synthetase (GS) expression in the liver and hippocampus and recognition memory in mice.
methodsAdult (5-month-old) and aged (15-month-old) male C57BL/6 mice were exposed to control diet (CD, 14% calories from fat) or HFD (60% fat). Novel place recognition testing was conducted and tissue was collected after 4 and 5 months on HFD, respectively. Tissue GS expression levels were assessed using immunohistochemistry and image analysis.
resultsThe obese mice developed moderate/severe hepatic steatosis. GS immunoreactivity was observed in perivenous hepatocytes and in hippocampal astrocytes and neuropil. Hepatic GS immunoreactivity density was higher in aged mice on HFD (n = 8) than CD (n = 13, P = 0.004). In aged mice, hippocampal GS immunoreactivity density was higher with HFD than CD (P = 0.037). In the novel place recognition test, aged mice were classified into impaired (n = 7) and unimpaired (n = 12), relative to adult mice (n = 22). Hippocampal GS immunoreactivity density was higher in impaired than unimpaired aged mice (P < 0.05).
conclusionLong-term exposure of aged mice to HFD was associated with increased GS expression in the liver and hippocampus. Novel place recognition impairment in aged mice was associated with increased hippocampal GS expression. These findings suggest that excess ammonia is involved in the age-related effects of HFD exposure and in neurotoxicity.
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