ArticlePlant molecular biology2016
Intron retention and rhythmic diel pattern regulation of carotenoid cleavage dioxygenase 2 during crocetin biosynthesis in saffron.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Isolation of Rhizospheric Fungi from Crocus sativus L. Rhizosphere and their Effects on Host-Growth Promotion.Current microbiology · 2026Article
- Intron Retention: A Reemerging Paradigm in RNA Biology and Post-Transcriptional Gene Regulation.Genes · 2025Review
- Montbretia flowers as a source of bioactive crocins: Biotechnology tools and delivery systems.Biotechnology reports (Amsterdam, Netherlands) · 2025Article
- Genome-wide analysis and identification of Carotenoid Cleavage Oxygenase (CCO) gene family in coffee (coffee arabica) under abiotic stress.BMC genomic data · 2024Article
- CstMYB1R1, a REVEILLE-8-like transcription factor, regulates diurnal clock-specific anthocyanin biosynthesis and response to abiotic stress in Crocus sativus L.Plant cell reports · 2023Article
- Genome-scale analysis of Arabidopsis splicing-related protein kinase families reveals roles in abiotic stress adaptation.BMC plant biology · 2022Article
- Subfunctionalization of D27 Isomerase Genes in Saffron.International journal of molecular sciences · 2022Article
- Metabolic Engineering of Crocin Biosynthesis inFrontiers in plant science · 2022Article
- Cloning and Prokaryotic Expression of Carotenoid Cleavage Dioxygenases from Mulberry (Evidence-based complementary and alternative medicine : eCAM · 2022Article
- A New Glycosyltransferase Enzyme from Family 91, UGT91P3, Is Responsible for the Final Glucosylation Step of Crocins in Saffron (International journal of molecular sciences · 2021Article
- The effect of salt stress on the production of apocarotenoids and the expression of genes related to their biosynthesis in saffron.Molecular biology reports · 2021Article
- Differential interaction of Or proteins with the PSY enzymes in saffron.Scientific reports · 2020Article
- Construction of a Stable and Temperature-Responsive Yeast Cell Factory for Crocetin Biosynthesis Using CRISPR-Cas9.Frontiers in bioengineering and biotechnology · 2020Article
- Carotenoid Cleavage Dioxygenases: Identification, Expression, and Evolutionary Analysis of This Gene Family in Tobacco.International journal of molecular sciences · 2019Article
- Multi-species transcriptome analyses for the regulation of crocins biosynthesis in Crocus.BMC genomics · 2019Article
- Article
- Transcriptome wide identification, phylogenetic analysis, and expression profiling of zinc-finger transcription factors from Crocus sativus L.Molecular genetics and genomics : MGG · 2017Article
- Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions.International journal of molecular sciences · 2016Review
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The carotenoid cleavage dioxygenase 2, a new member of the CCD family, catalyzes the conversion of zeaxanthin into crocetin-dialdehyde in Crocus. CCD2 is expressed in flowers, being responsible for the yellow, orange and red colorations displayed by tepals and stigma. Three CsCCD2 genes were identified in Crocus sativus, the longest contains ten exons and the shorter is a truncated copy with no introns and which lacks one exon sequence. Analysis of RNA-seq datasets of three developmental stages of saffron stigma allowed the determination of alternative splicing in CsCCD2, being intron retention (IR) the prevalent form of alternative splicing in CsCCD2. Further, high IR was observed in tissues that do not accumulate crocetin. The analysis of one CsCCD2 promoter showed cis-regulatory motifs involved in the response to light, temperature, and circadian regulation. The light and circadian regulation are common elements shared with the previously characterized CsLycB2a promoter, and these shared common cis-acting elements may represent binding sites for transcription factors responsible for co-regulation of these genes during the development of the stigma in saffron. A daily coordinated rhythmic regulation for CsCCD2 and CsLycB2a was observed, with higher levels of mRNA occurring at low temperatures during darkness, confirming the results obtained in the in silico promoter analysis. In addition, to the light and temperature dependent regulation of CsCCD2 expression, the apocarotenoid β-cyclocitral up-regulated CsCCD2 expression and could acts as a mediator of chromoplast-to-nucleus signalling, coordinating the expression of CsCCD2 with the developmental state of the chromoplast in the developing stigma.
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