ArticlePloS one2016
Comparison of RAPD, ISSR, and AFLP Molecular Markers to Reveal and Classify Orchardgrass (Dactylis glomerata L.) Germplasm Variations.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Traditional System Versus DNA Barcoding in Identification of Bamboo Species: A Systematic Review.Molecular biotechnology · 2021Pooled it
- Exploring genetic diversity and population structure of Turkish Dactylis glomerata L. germplasm using Start Codon Targeted (SCoT) marker system.BMC plant biology · 2026Article
- Evaluation of genetic diversity and elucidation of population structure of Lathyrus accessions using agro-morphological characters and ISSR technique.Journal, genetic engineering & biotechnology · 2025Article
- Genotyping-by-sequencing derived SNP markers reveal genetic diversity and population structure ofFrontiers in plant science · 2025Article
- Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China.BMC plant biology · 2024Article
- Association between molecular markers and resistance to bacterial blight using binary logistic analysis.BMC plant biology · 2024Article
- ISSR markers endorsed genetically stable plants regeneration of neem (Azadirachta indica A. Juss.) through indirect organogenesis using different explants.Molecular biology reports · 2023Article
- Genetic diversity and relationships among Nopalea sp. and Opuntia spp. accessions revealed by RAPD, ISSR and ITS molecular markers.Molecular biology reports · 2022Article
- EST-SSR Primer Development and Genetic Structure Analysis ofFrontiers in plant science · 2022Article
- Isolation and HPLC assisted quantification of two iridoid glycoside compounds and molecular DNA fingerprinting in critically endangered medicinalPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Amplified Fragment Length Polymorphism: Applications and Recent Developments.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Comparative assessment of ISSR, RAPD, and SCoT markers for genetic diversity in Clerodendrum species of North East India.Molecular biology reports · 2020Article
- Morphological Characterization and Genetic Diversity of Rice Blast Fungus,Journal of fungi (Basel, Switzerland) · 2020Article
- Deep learning can be used to train naïve, nonprofessional observers to detect diagnostic visual patterns of certain cancers in mammograms: a proof-of-principle study.Journal of medical imaging (Bellingham, Wash.) · 2020Article
- Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (The plant pathology journal · 2020Article
- Article
- Genetic diversity and population structure of watermelon (3 Biotech · 2019Article
- Molecular analysis of genetic diversity and population genetic structure inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Integrating morphological characters, molecular markers, and distribution patterns to assess the identity of Blepharis species from Jordan.Botanical studies · 2018Article
- Low genetic variation of invasiveEcology and evolution · 2018Article
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Authors and funding
5 authors.
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Abstract
Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The ISSR primers amplified 91 bands, and 54 showed polymorphism (59.3%). Finally, the AFLP showed 100 bands, of which 92 were polymorphic (92%). The fragments were scored as present (1) or absent (0), and those readings were entered in a computer file as a binary matrix (one for each marker). Three cluster analyses were performed to express--in the form of dendrograms--the relationships among the genotypes and the genetic variability detected. All DNA-based techniques used were able to amplify all of the genotypes. There were highly significant correlation coefficients between cophenetic matrices based on the genetic distance for the RAPD, ISSR, AFLP, and combined RAPD-ISSR-AFLP data (0.68, 0.78, 0.70, and 0.70, respectively). Two hypotheses were formulated to explain these results; both of them are in agreement with the results obtained using these three types of molecular markers. We conclude that when we study genotypes close related, the analysis of variability could require more than one DNA-based technique; in fact, the genetic variation present in different sources could interfere or combine with the more or less polymorphic ability, as our results showed for RAPD, ISSR and AFLP markers. Our results indicate that AFLP seemed to be the best-suited molecular assay for fingerprinting and assessing genetic relationship among genotypes of Dactylis glomerata.
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