Trial reportPloS one2016
Faecal Metaproteomic Analysis Reveals a Personalized and Stable Functional Microbiome and Limited Effects of a Probiotic Intervention in Adults.
Trial report in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Reshaping of the fecal proteome and metaproteome in obese patients 2 years after bariatric surgery.mSystems · 2026Trial
- Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants.Microbiome · 2018Trial
- Clinical Proteomics Applied to Food Allergy.Advances in experimental medicine and biology · 2026Review
- Identification of Key Features Pivotal to the Characteristics and Functions of Gut Bacteria Taxa through Machine Learning Methods.Current gene therapy · 2025Article
- Exploring the female genital tract mycobiome in young South African women using metaproteomics.Microbiome · 2025Article
- FISH-Flow Cytometry Reveals Microbiome-Wide Changes in Post-Translational Modification and Altered Microbial Abundance Among Children with Inflammatory Bowel Disease.Pathogens (Basel, Switzerland) · 2024Article
- Effects of Fermented Food Consumption on Non-Communicable Diseases.Foods (Basel, Switzerland) · 2023Review
- Gut Microbiome Proteomics in Food Allergies.International journal of molecular sciences · 2023Review
- Increasing taxonomic and functional characterization of host-microbiome interactions by DIA-PASEF metaproteomics.Frontiers in microbiology · 2023Article
- Introducing untargeted data-independent acquisition for metaproteomics of complex microbial samples.ISME communications · 2022Article
- Considerations for constructing a protein sequence database for metaproteomics.Computational and structural biotechnology journal · 2022Review
- ITS2 Sequencing and Targeted Meta-Proteomics of Infant Gut Mycobiome Reveal the Functional Role ofJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling.International journal of molecular sciences · 2021Review
- The Association Between Intestinal Bacteria and Allergic Diseases-Cause or Consequence?Frontiers in cellular and infection microbiology · 2021Review
- Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis.Frontiers in microbiology · 2021Article
- Diet influences the functions of the human intestinal microbiome.Scientific reports · 2020Article
- Nutraceuticals as modulators of gut microbiota: Role in therapy.British journal of pharmacology · 2020Review
- Synergistic effects of APOE and sex on the gut microbiome of young EFAD transgenic mice.Molecular neurodegeneration · 2019Article
- Development of omics-based protocols for the microbiological characterization of multi-strain formulations marketed as probiotics: the case of VSL#3.Microbial biotechnology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
Abstract
Recent metagenomic studies have demonstrated that the overall functional potential of the intestinal microbiome is rather conserved between healthy individuals. Here we assessed the biological processes undertaken in-vivo by microbes and the host in the intestinal tract by conducting a metaproteome analysis from a total of 48 faecal samples of 16 healthy adults participating in a placebo-controlled probiotic intervention trial. Half of the subjects received placebo and the other half consumed Lactobacillus rhamnosus GG for three weeks (1010 cfu per day). Faecal samples were collected just before and at the end of the consumption phase as well as after a three-week follow-up period, and were processed for microbial composition and metaproteome analysis. A common core of shared microbial protein functions could be identified in all subjects. Furthermore, we observed marked differences in expressed proteins between subjects that resulted in the definition of a stable and personalized microbiome both at the mass-spectrometry-based proteome level and the functional level based on the KEGG pathway analysis. No significant changes in the metaproteome were attributable to the probiotic intervention. A detailed taxonomic assignment of peptides and comparison to phylogenetic microarray data made it possible to evaluate the activity of the main phyla as well as key species, including Faecalibacterium prausnitzii. Several correlations were identified between human and bacterial proteins. Proteins of the human host accounted for approximately 14% of the identified metaproteome and displayed variations both between and within individuals. The individually different human intestinal proteomes point to personalized host-microbiota interactions. Our findings indicate that analysis of the intestinal metaproteome can complement gene-based analysis and contributes to a thorough understanding of the activities of the microbiome and the relevant pathways in health and disease.
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Registered trials
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