ArticleInternational journal of molecular sciences2016
Pathways Regulating Spheroid Formation of Human Follicular Thyroid Cancer Cells under Simulated Microgravity Conditions: A Genetic Approach.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- The Application of 3D Cell Culture in Thyroid Cancer.Annals of biomedical engineering · 2026Review
- Cellular response in three-dimensional spheroids and tissues exposed to real and simulated microgravity: a narrative review.NPJ microgravity · 2024Review
- Morphologic and genomic changes of thyroid cancer cell lines exposed to conditions of simulated microgravity.NPJ microgravity · 2024Article
- Omics Studies of Tumor Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Article
- Recent Advances in Breast Cancer Research.International journal of molecular sciences · 2023Article
- Prolonged Exposure to Simulated Microgravity Changes Release of Small Extracellular Vesicle in Breast Cancer Cells.International journal of molecular sciences · 2022Article
- Strategies to capitalize on cell spheroid therapeutic potential for tissue repair and disease modeling.NPJ Regenerative medicine · 2022Review
- The Fight against Cancer by Microgravity: The Multicellular Spheroid as a Metastasis Model.International journal of molecular sciences · 2022Review
- Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity.Frontiers in cell and developmental biology · 2022Article
- A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers.Bioengineered · 2021Article
- Changes in Exosomal miRNA Composition in Thyroid Cancer Cells after Prolonged Exposure to Real Microgravity in Space.International journal of molecular sciences · 2021Article
- Changes in Exosome Release in Thyroid Cancer Cells after Prolonged Exposure to Real Microgravity in Space.International journal of molecular sciences · 2021Article
- Survival Pathways Are Differently Affected by Microgravity in Normal and Cancerous Breast Cells.International journal of molecular sciences · 2021Article
- Mechanobiological Implications of Cancer Progression in Space.Frontiers in cell and developmental biology · 2021Review
- Effect of simulated microgravity on metabolism of HGC-27 gastric cancer cells.Oncology letters · 2020Article
- Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion-An Omics Network Approach.International journal of molecular sciences · 2020Article
- Dexamethasone Inhibits Spheroid Formation of Thyroid Cancer Cells Exposed to Simulated Microgravity.Cells · 2020Article
- Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.Scientific reports · 2019Article
- Fighting Thyroid Cancer with Microgravity Research.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microgravity induces three-dimensional (3D) growth in numerous cell types. Despite substantial efforts to clarify the underlying mechanisms for spheroid formation, the precise molecular pathways are still not known. The principal aim of this paper is to compare static 1g-control cells with spheroid forming (MCS) and spheroid non-forming (AD) thyroid cancer cells cultured in the same flask under simulated microgravity conditions. We investigated the morphology and gene expression patterns in human follicular thyroid cancer cells (UCLA RO82-W-1 cell line) after a 24 h-exposure on the Random Positioning Machine (RPM) and focused on 3D growth signaling processes. After 24 h, spheroid formation was observed in RPM-cultures together with alterations in the F-actin cytoskeleton. qPCR indicated more changes in gene expression in MCS than in AD cells. Of the 24 genes analyzed VEGFA, VEGFD, MSN, and MMP3 were upregulated in MCS compared to 1g-controls, whereas ACTB, ACTA2, KRT8, TUBB, EZR, RDX, PRKCA, CAV1, MMP9, PAI1, CTGF, MCP1 were downregulated. A pathway analysis revealed that the upregulated genes code for proteins, which promote 3D growth (angiogenesis) and prevent excessive accumulation of extracellular proteins, while genes coding for structural proteins are downregulated. Pathways regulating the strength/rigidity of cytoskeletal proteins, the amount of extracellular proteins, and 3D growth may be involved in MCS formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.