ReviewAllergo journal international
Molecular allergy diagnostics using multiplex assays: methodological and practical considerations for use in research and clinical routine: Part 21 of the Series Molecular Allergology.
Review in Allergo journal international. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- Microarray Analysis Confirms ImmunoCAP-Fluorescence Enzyme Immunoassay Results on Specific IgE in Patients with Atopic Dermatitis and Suspected Birch Pollen-Related Food Allergy.International archives of allergy and immunology · 2022Trial
- Exposome-driven molecular sensitization signatures in pediatric rhinitis and asthma across Spanish regions.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2026Observational
- A Fully Automated Chemiluminescent Immunoassay for Component-Resolved Diagnosis of Pollen Allergy: Validation of Six CHORUS CLIA Assays.Diagnostics (Basel, Switzerland) · 2026Article
- Molecular Diagnosis in Hymenoptera Allergy: Comparison of Euroline DPA-Dx and ImmunoCAP.Toxins · 2025Article
- Molecular Signatures of Aeroallergen Sensitization in Pediatric Populations: A Comparative Study Across Spanish Cities.International journal of molecular sciences · 2025Article
- A Comparison of asIgE Levels Measured with ALEX and ImmunoCAP ISAC in Polish Children with Food Allergies.International journal of molecular sciences · 2025Article
- Multiplex IgE peanut panels: a critical appraisal of assay designs and the good, the bad, and the ugly features of the applied allergen components.Frontiers in allergy · 2025Article
- Molecular Signatures of Aeroallergen Sensitization in Respiratory Allergy: A Comparative Study Across Climate-Matched Populations.International journal of molecular sciences · 2024Article
- Protein Biochips with Three-Dimensional Hydrogel or Polymer Brush Elements for the Detection of Human Serum Immunoglobulin E Specific to Inhalation Allergens.International journal of molecular sciences · 2024Article
- Multiplex Assays in Allergy Diagnosis: Allergy Explorer 2 versus ImmunoCAP ISAC E112i.Diagnostics (Basel, Switzerland) · 2024Article
- Selected Technical Aspects of Molecular Allergy Diagnostics.Current issues in molecular biology · 2023Review
- Intact Transition Epitope Mapping-Serological Inspection by Epitope EXtraction (ITEM-SIX).Molecules (Basel, Switzerland) · 2023Article
- Molecular allergy diagnosis is sensitive and avoids misdiagnosis in patients sensitized to seasonal allergens.Clinical and translational allergy · 2023Article
- Sensitization to molecular dog allergens in an adult population: Results from the West Sweden Asthma Study.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2023Article
- Diagnostic therapeutic care pathway for pediatric food allergies and intolerances in Italy: a joint position paper by the Italian Society for Pediatric Gastroenterology Hepatology and Nutrition (SIGENP) and the Italian Society for Pediatric Allergy and Immunology (SIAIP).Italian journal of pediatrics · 2022Review
- Integration of in vitro allergy test results and ratio analysis for the diagnosis and treatment of allergic patients (INTEGRA).Clinical and translational allergy · 2021Review
- Analysis of Results of Specific IgE in 100 Atopic Dermatitis Patients with the Use of Multiplex Examination ALEX2-Allergy Explorer.International journal of molecular sciences · 2021Article
- Atopic Dermatitis and Sensitisation to Molecular Components ofJournal of fungi (Basel, Switzerland) · 2021Article
- Pollen-food allergy syndrome and component sensitization in adolescents: A Japanese population-based study.PloS one · 2021Article
- VALIDA project: Validation of allergyAdvances in laboratory medicine · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The availability of single allergens and their use in microarray technology enables the simultaneous determination of specific IgE (sIgE) to a multitude of different allergens (> 100) in a multiplex procedure requiring only minute amounts of serum. This allows extensive individual sensitization profiles to be determined from a single analysis. Combined with a patient's medical history, these profiles simplify identification of cross-reactivity; permit a more accurate estimation of the risk of severe reactions; and enable the indication for specific immunotherapy to be more precisely established, particularly in cases of polysensitization. Strictly speaking, a multiplex assay is not a single test, but instead more than 100 simultaneous tests. This places considerable demands on the production, quality assurance, and interpretation of data. The following chapter describes the multiplex test systems currently available and discusses their characteristics. Performance data are presented and the sIgE values obtained from multiplex and singleplex assays are compared. Finally, the advantages and limitations of molecular allergy diagnostics using multiplex assays in clinical routine are discussed, and innovative possibilities for clinical research are described. The multiplex diagnostic tests available for clinical routine have now become well established. The interpretation of test results is demanding, particularly since all individual results need to be checked for their plausibility and clinical relevance on the basis of previous history (patient history, clinical symptoms, challenge test results). There is still room for improvement in certain areas, for example with respect to the overall test sensitivity of the method, as well as the availability and quality of particular allergens. The current test systems are just the beginning of a continuous development that will influence and most likely change clinical allergology in the coming years.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.