ArticlePeerJ2016
Improved ultrastructure of marine invertebrates using non-toxic buffers.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Morphological and functional characterization of the ptychocyte, a stingless stinging cell.bioRxiv : the preprint server for biology · 2026Article
- An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scales.bioRxiv : the preprint server for biology · 2026Article
- An ancient lysozyme in placozoans participates in acidic extracellular digestion.Communications biology · 2026Article
- The Antifouling Mechanism and Efficacy of Graphene Nanomaterials in Composite Coatings against Marine Diatoms.ACS omega · 2025Article
- An intranuclear bacterial parasite of deep-sea mussels expresses apoptosis inhibitors acquired from its host.Nature microbiology · 2024Article
- The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices.Scientific reports · 2024Article
- Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?Science advances · 2023Article
- Article
- Article
- Development and Characterization of Interstitial-Fluid-Mimicking Solutions for Pre-Clinical Assessment of Hypoxia.Diagnostics (Basel, Switzerland) · 2023Article
- Targeted volume correlative light and electron microscopy of an environmental marine microorganism.Journal of cell science · 2023Article
- DAPI staining and DNA content estimation of nuclei in uncultivable microbial eukaryotes (Arcellinida and Ciliates).European journal of protistology · 2021Article
- High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue.Frontiers in immunology · 2021Article
- Two intracellular and cell type-specific bacterial symbionts in the placozoan Trichoplax H2.Nature microbiology · 2019Article
- Fueled by methane: deep-sea sponges from asphalt seeps gain their nutrition from methane-oxidizing symbionts.The ISME journal · 2019Article
- Article
- A Practical Approach to Study Uncultivated Protists Using Single-Cell Techniques for Electron Microscopy.The Journal of eukaryotic microbiologyArticle
- Characterization of Allobodo yubaba sp. nov. and Novijibodo darinka gen. et sp. nov., cultivable free-living species of the phylogenetically enigmatic kinetoplastid taxon Allobodonidae.The Journal of eukaryotic microbiologyArticle
- Ultrastructure of Olkasia polycarbonata (Euglenozoa, Euglenida) demonstrates cytoskeletal innovations associated with the feeding and flagellar apparatuses.The Journal of eukaryotic microbiologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Many marine biology studies depend on field work on ships or remote sampling locations where sophisticated sample preservation techniques (e.g., high-pressure freezing) are often limited or unavailable. Our aim was to optimize the ultrastructural preservation of marine invertebrates, especially when working in the field. To achieve chemically-fixed material of the highest quality, we compared the resulting ultrastructure of gill tissue of the mussel Mytilus edulis when fixed with differently buffered EM fixatives for marine specimens (seawater, cacodylate and phosphate buffer) and a new fixative formulation with the non-toxic PHEM buffer (PIPES, HEPES, EGTA and MgCl2). All buffers were adapted for immersion fixation to form an isotonic fixative in combination with 2.5% glutaraldehyde. We showed that PHEM buffer based fixatives resulted in equal or better ultrastructure preservation when directly compared to routine standard fixatives. These results were also reproducible when extending the PHEM buffered fixative to the fixation of additional different marine invertebrate species, which also displayed excellent ultrastructural detail. We highly recommend the usage of PHEM-buffered fixation for the fixation of marine invertebrates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.