ArticleMolecular biology international2016
SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha.
Article in Molecular biology international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- An overview of remote monitoring methods in biodiversity conservation.Environmental science and pollution research international · 2022Review
- Review
- Article
- Development of an In Vitro Propagation Protocol and a Sequence Characterized Amplified Region (SCAR) Marker ofPlants (Basel, Switzerland) · 2020Article
- Development of Species-Specific SCAR Markers, Based on a SCoT Analysis, to AuthenticateFrontiers in genetics · 2018Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Commercially important Commiphora species are drought-tolerant plants and they are leafless for most of the year. Therefore, it is necessary to develop some molecular marker for the identification. Intended for that, in the present study, species-specific, sequence-characterized amplified regions (SCAR) markers were developed for proficient and precise identification of closely related species Commiphora wightii and C. myrrha, which may ensure the quality, safety, and efficacy of medicines made from these plants through adulterous mixing of these plants. Two species-specific RAPD amplicons were selected, gel-purified, cloned, and sequenced after screening of 20 RAPD primers. The sequence of 979 and 590 nucleotides (Genebank accession numbers K90051 and K90052) was used for development of 4 SCAR markers, namely, Sc1P, Sc1Pm, Sc2P, and Sc2Pm. Out of them, the Sc1Pm was specific for C. wightii, while Sc2P discriminated both the Commiphora species. These markers are first reported and will be useful for rapid identification of closely related Commiphora wightii and C. myrrha species.
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