Evidence map›Paper›PMID 27069482›Full record

ArticleStem cells international2016

Naringin Stimulates Osteogenic Differentiation of Rat Bone Marrow Stromal Cells via Activation of the Notch Signaling Pathway.

Guo-Yong Yu, Gui-Zhou Zheng, Bo Chang, Qin-Xiao Hu, Fei-Xiang Lin, De-Zhong Liu, Chu-Cheng Wu, Shi-Xin Du, Xue-Dong Li

RetractedOpen access · goldAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. The Role of Herbal Medicine in Modulating Bone Homeostasis.Current topics in medicinal chemistry · 2024
    Review
  5. Article
  6. Review
  7. Article
  8. The potential of plant extracts in cell therapy.Stem cell research & therapy · 2022
    Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Guo-Yong YuDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China.
Gui-Zhou ZhengDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China.
Bo ChangDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China.
Qin-Xiao HuDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China.
Fei-Xiang LinDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China.
De-Zhong LiuDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China.
Chu-Cheng WuDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China.
Shi-Xin DuDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China; Department of Orthopedics, The Affiliated Luohu Hospital of Shenzhen University, Shenzhen, Guangdong Province 518000, China.
Xue-Dong LiDepartment of Orthopedics, The First Affiliated Hospital, Shantou University Medical College, 57 Changping Road, Shantou, Guangdong Province 515041, China; Department of Orthopedics, The Affiliated Luohu Hospital of Shenzhen University, Shenzhen, Guangdong Province 518000, China.ORCID 0000-0001-6482-9369
Shantou University · CNShenzhen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Naringin is a major flavonoid found in grapefruit and is an active compound extracted from the Chinese herbal medicine Rhizoma Drynariae. Naringin is a potent stimulator of osteogenic differentiation and has potential application in preventing bone loss. However, the signaling pathway underlying its osteogenic effect remains unclear. We hypothesized that the osteogenic activity of naringin involves the Notch signaling pathway. Rat bone marrow stromal cells (BMSCs) were cultured in osteogenic medium containing-naringin, with or without DAPT (an inhibitor of Notch signaling), the effects on ALP activity, calcium deposits, osteogenic genes (ALP, BSP, and cbfa1), adipogenic maker gene PPARγ2 levels, and Notch expression were examined. We found that naringin dose-dependently increased ALP activity and Alizarin red S staining, and treatment at the optimal concentration (50 μg/mL) increased mRNA levels of osteogenic genes and Notch1 expression, while decreasing PPARγ2 mRNA levels. Furthermore, treatment with DAPT partly reversed effects of naringin on BMSCs, as judged by decreases in naringin-induced ALP activity, calcium deposits, and osteogenic genes expression, as well as upregulation of PPARγ2 mRNA levels. These results suggest that the osteogenic effect of naringin partly involves the Notch signaling pathway.

Identifiers

PMID27069482
PMCPMC4812486
OpenAlexW2296897173

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.