ArticleJournal of experimental botany2016
Arabidopsis ubiquitin-conjugating enzyme UBC22 is required for female gametophyte development and likely involved in Lys11-linked ubiquitination.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Characterization and expression analysis of UBC gene family provide insights into the potential roles in pigment biosynthesis in pepper fruit.BMC genomics · 2026Article
- The Ubiquitin-Proteasome System in Flowering Plant Reproduction: Mechanisms, Functional Diversity, and Regulatory Networks.Plants (Basel, Switzerland) · 2026Review
- Insights into double sigmoid pattern during Chinese cherry fruit development through comparative physiological and transcriptomic profiles.BMC plant biology · 2025Article
- Genome-wide identification of wheat Ubiquitin-conjugating enzyme gene family and its role in wheat powdery mildew disease resistance.BMC plant biology · 2025Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- A dynamic ubiquitination balance of cell proliferation and endoreduplication regulators determines plant organ size.Science advances · 2024Article
- Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield ofFrontiers in plant science · 2023Article
- BSR and Full-Length Transcriptome Approaches Identified Candidate Genes for High Seed Ratio inCurrent issues in molecular biology · 2022Article
- In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay.Bio-protocol · 2022Article
- Arabidopsis Ubiquitin-Conjugating Enzymes UBC4, UBC5, and UBC6 Have Major Functions in Sugar Metabolism and Leaf Senescence.International journal of molecular sciences · 2022Article
- Harnessing the ubiquitin code to respond to environmental cues.Essays in biochemistry · 2022Article
- Deciphering the Host-Pathogen Interactome of the Wheat-Common Bunt System: A Step towards Enhanced Resilience in Next Generation Wheat.International journal of molecular sciences · 2022Article
- Characterization and regulation mechanism analysis of ubiquitin-conjugating family genes in strawberry reveals a potential role in fruit ripening.BMC plant biology · 2022Article
- Deciphering the Crosstalk Mechanisms of Wheat-Stem Rust Pathosystem: Genome-Scale Prediction Unravels Novel Host Targets.Frontiers in plant science · 2022Article
- The Inactivation of ArabidopsisPlants (Basel, Switzerland) · 2021Article
- Genome-wide association study and transcriptome analysis dissect the genetic control of silique length in Brassica napus L.Biotechnology for biofuels · 2021Article
- Global identification of long non-coding RNAs involved in the induction of spinach flowering.BMC genomics · 2021Article
- Beyond K48 and K63: non-canonical protein ubiquitination.Cellular & molecular biology letters · 2021Review
- Ubiquitination in Plant Meiosis: Recent Advances and High Throughput Methods.Frontiers in plant science · 2021Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein ubiquitination is critical for numerous processes in eukaryotes. The ubiquitin-conjugating enzyme (E2) is required for ubiquitination. The Arabidopsis genome has approximately 37 E2 genes, but in vivo functions for most of them remain unknown. In this study we observed that knockout mutants of Arabidopsis UBC22 had much-reduced silique length and seed number, with nearly 90% of ovules aborted. Analyses revealed that the majority of mutant embryo sacs displayed severe defects and often contained no gamete nuclei. There was no difference between mutant and wild-type Arabidopsis at the megaspore mother cell stage; however, the functional megaspore was either not present or appeared abnormal in a large portion of mutant ovules, suggesting that the defect started with functional megaspore degeneration in the mutants. Degeneration continued during megagametogenesis, such that the percentage of mature embryo sacs without any gamete nuclei was much greater than the percentage of developing ovules without a functional megaspore and, in addition, various abnormalities in megagametogenesis were observed. Additionally, heterozygous plants had only 13.1% of ovules aborted, indicating that the heterozygous sporophytic tissues could affect the development of the mutant female gametophyte. UBC22 is the sole member of an Arabidopsis E2 subfamily, and is more closely related to one type of E2s in animals that catalyzes Lys11-specific ubiquitination. Indeed, our results showed that Arabidopsis UBC22 could catalyze ubiquitin dimer formation in vitro in a Lys11-dependent manner, suggesting that it likely catalyzes Lys11-linked ubiquitination in plants. This study has thus identified one biochemical property of UBC22 and revealed a novel function in female gametophyte development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.