ArticleMolecular pain2016
Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord.
Article in Molecular pain, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Tac1 deficiency reduces the severity of enteric bacterial infection.PLoS pathogens · 2026Article
- Article
- The contribution of endocytic mediators to itch transmission.bioRxiv : the preprint server for biology · 2026Article
- The contribution of endocytic mediators to itch transmission.Frontiers in molecular neuroscience · 2026Article
- Pruritus: A Sensory Symptom Generated in Cutaneous Immuno-Neuronal Crosstalk.Frontiers in pharmacology · 2022Review
- A neuropeptide code for itch.Nature reviews. Neuroscience · 2021Review
- Chemogenetic activation of central gastrin-releasing peptide-expressing neurons elicits itch-related scratching behavior in male and female mice.Pharmacology research & perspectives · 2021Article
- Spinal Cord Injury Causes Reduction ofFrontiers in neurology · 2021Article
- Central circuit mechanisms of itch.Nature communications · 2020Review
- GRP receptor and AMPA receptor cooperatively regulate itch-responsive neurons in the spinal dorsal horn.Neuropharmacology · 2020Article
- Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors.Nature communications · 2020Article
- Interventions in the B-type natriuretic peptide signalling pathway as a means of controlling chronic itch.British journal of pharmacology · 2020Review
- Pain Inhibits GRPR Neurons via GABAergic Signaling in the Spinal Cord.Scientific reports · 2019Article
- Cross-talk between Human Spinal Cord μ-opioid Receptor 1Y Isoform and Gastrin-releasing Peptide Receptor Mediates Opioid-induced Scratching Behavior.Anesthesiology · 2019Article
- Peripheral and Central Mechanisms of Itch.Neuron · 2018Review
- Non-canonical Opioid Signaling Inhibits Itch Transmission in the Spinal Cord of Mice.Cell reports · 2018Article
- Spinal Mechanisms of Itch Transmission.Neuroscience bulletin · 2018Review
- Distinct roles of NMB and GRP in itch transmission.Scientific reports · 2017Article
- Molecular and neural basis of contagious itch behavior in mice.Science (New York, N.Y.) · 2017Article
- Comparative Anatomy of Gastrin-releasing Peptide Pathways in the Trigeminal Sensory System of Mouse and the Asian House Musk ShrewActa histochemica et cytochemica · 2016Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
There are substantial disagreements about the expression of gastrin-releasing peptide (GRP) in sensory neurons and whether GRP antibody cross-reacts with substance P (SP). These concerns necessitate a critical revaluation of GRP expression using additional approaches. Here, we show that a widely used GRP antibody specifically recognizes GRP but not SP. In the spinal cord of mice lacking SP (Tac1KO), the expression of not only GRP but also other peptides, notably neuropeptide Y (NPY), is significantly diminished. We detectedGrpmRNA in dorsal root ganglias using reverse transcription polymerase chain reaction, in situ hybridization and RNA-seq. We demonstrated thatGrpmRNA and protein are upregulated in dorsal root ganglias, but not in the spinal cord, of mice with chronic itch. Few GRP(+)immunostaining signals were detected in spinal sections following dorsal rhizotomy and GRP(+)cell bodies were not detected in dissociated dorsal horn neurons. Ultrastructural analysis further shows that substantially more GRPergic fibers form synaptic contacts with gastrin releasing peptide receptor-positive (GRPR(+)) neurons than SPergic fibers. Our comprehensive study demonstrates that a majority of GRPergic fibers are of primary afferent origin. A number of factors such as low copy number ofGrptranscripts, small percentage of cells expressingGrp, and the use of an eGFP GENSAT transgenic as a surrogate for GRP protein have contributed to the controversy. Optimization of experimental procedures facilitates the specific detection of GRP expression in dorsal root ganglia neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.