ArticleBMC plant biology2016
Identification of miRNAs and their targets through high-throughput sequencing and degradome analysis in male and female Asparagus officinalis.
Article in BMC plant biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Non-coding RNAs as central regulators of phytohormone signaling in plant abiotic stress responses.Plant molecular biology · 2026Review
- Functional role of microRNA in the regulation of biotic and abiotic stress in agronomic plants.Frontiers in genetics · 2023Review
- Identification of miRNAs and Target Genes at Key Stages of Sexual Differentiation in AndrodioeciousInternational journal of molecular sciences · 2022Article
- Identification of Known and NovelLife (Basel, Switzerland) · 2022Article
- Integrative Analyses of Transcriptomics and Metabolomics in Sex Differentiation of Mulberry Flowers.Frontiers in molecular biosciences · 2022Article
- The emerging role of small RNAs in ovule development, a kind of magic.Plant reproduction · 2021Review
- Article
- Uncovering anthocyanin biosynthesis related microRNAs and their target genes by small RNA and degradome sequencing in tuberous roots of sweetpotato.BMC plant biology · 2019Article
- Transcriptome analysis of miRNAs expression reveals novel insights into adventitious root formation in lotus (Nelumbo nucifera Gaertn.).Molecular biology reports · 2019Article
- Integrated microRNA and transcriptome profiling reveals a miRNA-mediated regulatory network of embryo abortion under calcium deficiency in peanut (Arachis hypogaea L.).BMC genomics · 2019Article
- Sex and the flower - developmental aspects of sex chromosome evolution.Annals of botany · 2018Review
- Non-coding RNAs and plant male sterility: current knowledge and future prospects.Plant cell reports · 2018Review
- Article
- Comparative transcriptome analysis reveals differentially expressed genes associated with sex expression in garden asparagus (Asparagus officinalis).BMC plant biology · 2017Article
- Water-deficit stress-responsive microRNAs and their targets in four durum wheat genotypes.Functional & integrative genomics · 2017Article
- De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris.BMC plant biology · 2017Article
- Transcriptome-Wide Analysis ofFrontiers in plant science · 2017Article
- Genome-Wide Identification of MicroRNAs and Their Targets in the Leaves and Fruits ofFrontiers in plant science · 2016Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs), a class of non-coding small RNAs (sRNAs), regulate various biological processes. Although miRNAs have been identified and characterized in several plant species, miRNAs in Asparagus officinalis have not been reported. As a dioecious plant with homomorphic sex chromosomes, asparagus is regarded as an important model system for studying mechanisms of plant sex determination.
resultsTwo independent sRNA libraries from male and female asparagus plants were sequenced with Illumina sequencing, thereby generating 4.13 and 5.88 million final clean reads, respectively. Both libraries predominantly contained 24-nt sRNAs, followed by 21-nt sRNAs. Further analysis identified 154 conserved miRNAs, which belong to 26 families, and 39 novel miRNA candidates seemed to be specific to asparagus. Comparative profiling revealed that 63 miRNAs exhibited significant differential expression between male and female plants, which was confirmed by real-time quantitative PCR analysis. Among them, 37 miRNAs were significantly up-regulated in the female library, whereas the others were preferentially expressed in the male library. Furthermore, 40 target mRNAs representing 44 conserved and seven novel miRNAs were identified in asparagus through high-throughput degradome sequencing. Functional annotation showed that these target mRNAs were involved in a wide range of developmental and metabolic processes.
conclusionsWe identified a large set of conserved and specific miRNAs and compared their expression levels between male and female asparagus plants. Several asparagus miRNAs, which belong to the miR159, miR167, and miR172 families involved in reproductive organ development, were differentially expressed between male and female plants, as well as during flower development. Consistently, several predicted targets of asparagus miRNAs were associated with floral organ development. These findings suggest the potential roles of miRNAs in sex determination and reproductive developmental processes in asparagus.
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