Evidence map›Paper›PMID 27067053›Full record

ArticleMolecular & cellular proteomics : MCP2016

N-Glycopeptide Profiling in Arabidopsis Inflorescence.

Shou-Ling Xu, Katalin F Medzihradszky, Zhi-Yong Wang, Alma L Burlingame, Robert J Chalkley

Abstract read
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Plants (Basel, Switzerland) · 2022
    Review
  2. Review
  3. Review
  4. Article
  5. Cracking the "Sugar Code": A Snapshot ofFrontiers in plant science · 2021
    Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Molecular & cellular proteomics : MCP · 2018
    Article
  11. Review
  12. Article
  13. Review
  14. Proteomic analysis reveals O-GlcNAc modification on proteins with key regulatory functions inProceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  15. Article
  16. Chemical Glycoproteomics.Chemical reviews · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shou-Ling XuFrom the ‡Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305; §Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143.
Katalin F Medzihradszky§Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143.
Zhi-Yong WangFrom the ‡Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305;
Alma L Burlingame§Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143.
Robert J Chalkley§Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143 chalkley@cgl.ucsf.edu.

Funding

Bio-Organic Biomedical Mass Spectromy ResourceP41GM103481 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BURLINGAME, ALMA L · 2012 to 2015
$6.4M
Howard Hughes Medical InstituteNIGMS NIH HHS P41 GM103481
6 · The paper itself

Abstract

This study presents the first large-scale analysis of plant intact glycopeptides. Using wheat germ agglutinin lectin weak affinity chromatography to enrich modified peptides, followed by electron transfer dissociation (ETD)(1) fragmentation tandem mass spectrometry, glycan compositions on over 1100 glycopeptides from 270 proteins found in Arabidopsis inflorescence tissue were characterized. While some sites were only detected with a single glycan attached, others displayed up to 16 different glycoforms. Among the identified glycopeptides were four modified in nonconsensus glycosylation motifs. While most of the modified proteins are secreted, membrane, endoplasmic reticulum (ER), or Golgi-localized proteins, surprisingly, N-linked sugars were detected on a protein predicted to be cytosolic or nuclear.

Indexed as

ArabidopsisArabidopsis ProteinsCell NucleusChromatography, LiquidCytosolGlycopeptidesGlycosylationInflorescenceProtein Processing, Post-TranslationalTandem Mass SpectrometryArabidopsis ProteinsGlycopeptides

Identifiers

PMID27067053
PMCPMC5083099

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.