ArticleNature chemical biology2016
Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking.
Article in Nature chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- Light-induced spindle assembly through Aurora A clustering.EMBO reports · 2026Article
- Cold exposure-inducedActa pharmaceutica Sinica. B · 2026Article
- Functionally Essential and Structurally Diverse: Insights into the zebrafish Left-Right Organizer's Cilia via Optogenetic IFT88 Perturbation and Volume Electron Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Programmable solid-state condensates for spatiotemporal control of mammalian gene expression.Nature chemical biology · 2025Article
- Spatiotemporal control of subcellular O-GlcNAc signaling using Opto-OGT.Nature chemical biology · 2025Article
- Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- In vivo optogenetic manipulations of endogenous proteins reveal spatiotemporal roles of microtubule and kinesin in dendrite patterning.Science advances · 2024Article
- Propofol improves survival in a murine model of sepsis via inhibiting Rab5a-mediated intracellular trafficking of TLR4.Journal of translational medicine · 2024Article
- Molecular mechanisms of lipid droplets-mitochondria coupling in obesity and metabolic syndrome: insights and pharmacological implications.Frontiers in physiology · 2024Review
- PLC-γ-CaFrontiers in molecular neuroscience · 2024Article
- Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development.PLoS genetics · 2023Article
- Article
- Understanding the interplay of membrane trafficking, cell surface mechanics, and stem cell differentiation.Seminars in cell & developmental biology · 2023Review
- The emergence of molecular systems neuroscience.Molecular brain · 2022Review
- Optogenetic Control of Membrane Trafficking Using Light-Activated Reversible Inhibition by Assembly Trap of Intracellular Membranes (IM-LARIAT).Methods in molecular biology (Clifton, N.J.) · 2022Article
- CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditis elegans.Genetics · 2021Article
- Deliver on Time or Pay the Fine: Scheduling in Membrane Trafficking.International journal of molecular sciences · 2021Review
- mem-iLID, a fast and economic protein purification method.Bioscience reports · 2021Article
- Review
- Optogenetics: The Art of Illuminating Complex Signaling Pathways.Physiology (Bethesda, Md.) · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular membrane trafficking, which is involved in diverse cellular processes, is dynamic and difficult to study in a spatiotemporal manner. Here we report an optogenetic strategy, termed light-activated reversible inhibition by assembled trap of intracellular membranes (IM-LARIAT), that uses various Rab GTPases combined with blue-light-induced hetero-interaction between cryptochrome 2 and CIB1. In this system, illumination induces a rapid and reversible intracellular membrane aggregation that disrupts the dynamics and functions of the targeted membrane. We applied IM-LARIAT to specifically perturb several Rab-mediated trafficking processes, including receptor transport, protein sorting and secretion, and signaling initiated from endosomes. We finally used this tool to reveal different functions of local Rab5-mediated and Rab11-mediated membrane trafficking in growth cones and soma of young hippocampal neurons. Our results show that IM-LARIAT is a versatile tool that can be used to dissect spatiotemporal functions of intracellular membranes in diverse systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.