ArticleBiochemistry2016
S-Nitrosation of Conserved Cysteines Modulates Activity and Stability of S-Nitrosoglutathione Reductase (GSNOR).
Article in Biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 120 citations in OpenAlex.
- Modulation of GSNOR activity for improved NO homeostasis and flood resilience in plants.Plant signaling & behavior · 2026Review
- Integration of NO and HPlant communications · 2026Review
- GSNOR is essential for nitric oxide homeostasis and involved in aflatoxin biosynthesis and pathogenicity inApplied and environmental microbiology · 2026Article
- Phytophthora Disrupts Plant Immunity by Manipulating Nitric Oxide Homeostasis Through GSNOR Inhibition.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Nitro-fatty acids modulate germination onset through S-nitrosothiol metabolism.Plant physiology · 2025Article
- Post-Translational Modifications to Cysteine Residues in Plant Proteins and Their Impact on the Regulation of Metabolism and Signal Transduction.International journal of molecular sciences · 2024Review
- Regulation of nitro-oxidative homeostasis: an effective approach to enhance salinity tolerance in plants.Plant cell reports · 2024Review
- De-nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Persulfidome of Sweet Pepper Fruits during Ripening: The Case Study of Leucine Aminopeptidase That Is Positively Modulated by HAntioxidants (Basel, Switzerland) · 2024Article
- Maternal nitric oxide homeostasis impacts female gametophyte development under optimal and stress conditions.The Plant cell · 2024Article
- Reactive oxygen species- and nitric oxide-dependent regulation of ion and metal homeostasis in plants.Journal of experimental botany · 2023Article
- Functions of NO and HMethods in molecular biology (Clifton, N.J.) · 2023Article
- Focus on Nitric Oxide Homeostasis: Direct and Indirect Enzymatic Regulation of Protein Denitrosation Reactions in Plants.Antioxidants (Basel, Switzerland) · 2022Review
- Thiol-based Oxidative Posttranslational Modifications (OxiPTMs) of Plant Proteins.Plant & cell physiology · 2022Review
- Molecular dissection of a dedicated formaldehyde dehydrogenase from Mycobacterium smegmatis.Protein science : a publication of the Protein Society · 2022Article
- Redox post-translational modifications and their interplay in plant abiotic stress tolerance.Frontiers in plant science · 2022Review
- Can NO Signaling and Its Metabolism Be Used to Improve Nutrient Use Efficiency? Toward a Research Agenda.Frontiers in plant science · 2022Article
- Molecular responses of legumes to abiotic stress: post-translational modifications of proteins and redox signaling.Journal of experimental botany · 2021Review
- Crosstalk between abscisic acid and nitric oxide under heat stress: exploring new vantage points.Plant cell reports · 2021Review
- PodocyteFrontiers in pharmacology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The free radical nitric oxide (NO(•)) regulates diverse physiological processes from vasodilation in humans to gas exchange in plants. S-Nitrosoglutathione (GSNO) is considered a principal nitroso reservoir due to its chemical stability. GSNO accumulation is attenuated by GSNO reductase (GSNOR), a cysteine-rich cytosolic enzyme. Regulation of protein nitrosation is not well understood since NO(•)-dependent events proceed without discernible changes in GSNOR expression. Because GSNORs contain evolutionarily conserved cysteines that could serve as nitrosation sites, we examined the effects of treating plant (Arabidopsis thaliana), mammalian (human), and yeast (Saccharomyces cerevisiae) GSNORs with nitrosating agents in vitro. Enzyme activity was sensitive to nitroso donors, whereas the reducing agent dithiothreitol (DTT) restored activity, suggesting that catalytic impairment was due to S-nitrosation. Protein nitrosation was confirmed by mass spectrometry, by which mono-, di-, and trinitrosation were observed, and these signals were sensitive to DTT. GSNOR mutants in specific non-zinc-coordinating cysteines were less sensitive to catalytic inhibition by nitroso donors and exhibited reduced nitrosation signals by mass spectrometry. Nitrosation also coincided with decreased tryptophan fluorescence, increased thermal aggregation propensity, and increased polydispersity-properties reflected by differential solvent accessibility of amino acids important for dimerization and the shape of the substrate and coenzyme binding pockets as assessed by hydrogen-deuterium exchange mass spectrometry. Collectively, these data suggest a mechanism for NO(•) signal transduction in which GSNOR nitrosation and inhibition transiently permit GSNO accumulation.
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