ArticleFrontiers in plant science2016
Biochemical and Molecular Characterization of a Flavonoid 3-O-glycosyltransferase Responsible for Anthocyanins and Flavonols Biosynthesis in Freesia hybrida.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 87 citations in OpenAlex.
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- Analysis of differential mechanisms in flavonoid synthesis in the exocarp of virescens and nigrescens fruits and development of KASP molecular markers.BMC plant biology · 2026Article
- Two Indel Variations in the Regulated Carotenoid Pigment Gene Hold the Key to the Interspecies Flower Colour Differentiation in Liriodendron Plants.Plant biotechnology journal · 2026Article
- The role of RaDFR2 regulating flower color formation in Rhododendron agastum.BMC plant biology · 2026Article
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- Identification of the UGT Family and Functional Validation ofPlants (Basel, Switzerland) · 2025Article
- Genome-wide identification of the UGT genes family in Acer rubrum and role of ArUGT52 in anthocyanin biosynthesis under cold stress.BMC plant biology · 2025Article
- Natural variations in the Cis-elements of GhRPRS1 contributing to petal colour diversity in cotton.Plant biotechnology journal · 2024Article
- A multiscale approach to understanding the shared blue-orange flower color polymorphism in two Lysimachia species.BMC plant biology · 2024Article
- Analysis of Active Components and Transcriptome ofInternational journal of molecular sciences · 2024Article
- Highly Promiscuous Flavonoid Di-Molecules (Basel, Switzerland) · 2024Article
- Cloned genes and genetic regulation of anthocyanin biosynthesis in maize, a comparative review.Frontiers in plant science · 2024Review
- Comparative genome and metabolome analyses uncover the evolution and flavonoid biosynthesis betweeniScience · 2023Article
- Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production.Frontiers in plant science · 2023Article
- The SARS-CoV-2 main protease (MMedComm · 2022Review
- Characterization of Two Key Flavonoid 3-Frontiers in plant science · 2022Article
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9 authors at 1 institution in 1 country.
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Abstract
The glycosylation of flavonoids increases their solubility and stability in plants. Flowers accumulate anthocyanidin and flavonol glycosides which are synthesized by UDP-sugar flavonoid glycosyltransferases (UFGTs). In our previous study, a cDNA clone (Fh3GT1) encoding UFGT was isolated from Freesia hybrida, which was preliminarily proved to be invovled in cyanidin 3-O-glucoside biosynthesis. Here, a variety of anthocyanin and flavonol glycosides were detected in flowers and other tissues of F. hybrida, implying the versatile roles of Fh3GT1 in flavonoids biosynthesis. To further unravel its multi-functional roles, integrative analysis between gene expression and metabolites was investigated. The results showed expression of Fh3GT1 was positively related to the accumulation of anthocyanins and flavonol glycosides, suggesting its potential roles in the biosynthesis of both flavonoid glycosides. Subsequently, biochemical analysis results revealed that a broad range of flavonoid substrates including flavonoid not naturally occurred in F. hybrida could be recognized by the recombinant Fh3GT1. Both UDP-glucose and UDP-galactose could be used as sugar donors by recombinant Fh3GT1, although UDP-galactose was transferred with relatively low activity. Furthermore, regiospecificity analysis demonstrated that Fh3GT1 was able to glycosylate delphinidin at the 3-, 4-', and 7- positions in a sugar-dependent manner. And the introduction of Fh3GT1 into Arabidopsis UGT78D2 mutant successfully restored the anthocyanins and flavonols phenotypes caused by lost-of-function of the 3GT, indicating that Fh3GT1 functions as a flavonoid 3-O-glucosyltransferase in vivo. In summary, these results demonstrate that Fh3GT1 is a flavonoid 3-O-glycosyltransferase using UDP-glucose as the preferred sugar donor and may involve in flavonoid glycosylation in F. hybrida.
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