ArticleBioMed research international2016
Overexpression of AaWRKY1 Leads to an Enhanced Content of Artemisinin in Artemisia annua.
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Regulatory architecture of high-altitude adaptation in Artemisia: from signal perception to specialized metabolism.Plant cell reports · 2026Review
- PnWRKY38-PnSUS1 axis regulates the biosynthesis ofHorticulture research · 2026Article
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- Salicylic acid regulates biosynthesis of floral fragrance (E)-β-farnesene via NPR3-WRKY1 module in chrysanthemum.Molecular horticulture · 2025Article
- Metabolic Engineering of Terpenoid Biosynthesis in Medicinal Plants: From Genomic Insights to Biotechnological Applications.Current issues in molecular biology · 2025Review
- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- The PlMYB73-PlMYB70-PlMYB108 complex regulatesHorticulture research · 2025Article
- HMGR Modulates Strawberry Fruit Coloration and Aroma Through Regulating Terpenoid and Anthocyanin Pathways.Foods (Basel, Switzerland) · 2025Article
- Comprehensive characterization of the WRKY gene family and their potential roles in regulation phenylphenalenone biosynthesis inFrontiers in plant science · 2025Article
- A transcription factor of SHI family AaSHI1 activates artemisinin biosynthesis genes in Artemisia annua.BMC genomics · 2024Article
- Chemically Mediated Plant-Plant Interactions: Allelopathy and Allelobiosis.Plants (Basel, Switzerland) · 2024Review
- Advanced metabolic engineering strategies for increasing artemisinin yield inHorticulture research · 2024Article
- Genome-wide investigation of WRKY gene family inFrontiers in plant science · 2024Article
- Transcriptome analysis reveals regulatory mechanism of methyl jasmonate-induced monoterpenoid biosynthesis inFrontiers in plant science · 2024Article
- WRKY Transcription Factors inBiomolecules · 2023Article
- Molecular Cloning and Characterization of WRKY12, A Pathogen Induced WRKY Transcription Factor fromGenes · 2023Article
- Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023Article
- From Plant to Yeast-Advances in Biosynthesis of Artemisinin.Molecules (Basel, Switzerland) · 2022Review
- Article
- Natural products of medicinal plants: biosynthesis and bioengineering in post-genomic era.Horticulture research · 2022Article
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Authors and funding
13 authors.
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Abstract
Artemisinin is an effective component of drugs against malaria. The regulation of artemisinin biosynthesis is at the forefront of artemisinin research. Previous studies showed that AaWRKY1 can regulate the expression of ADS, which is the first key enzyme in artemisinin biosynthetic pathway. In this study, AaWRKY1 was cloned, and it activated ADSpro and CYPpro in tobacco using dual-LUC assay. To further study the function of AaWRKY1, pCAMBIA2300-AaWRKY1 construct under 35S promoter was generated. Transgenic plants containing AaWRKY1 were obtained, and four independent lines with high expression of AaWRKY1 were analyzed. The expression of ADS and CYP, the key enzymes in artemisinin biosynthetic pathway, was dramatically increased in AaWRKY1-overexpressing A. annua plants. Furthermore, the artemisinin yield increased significantly in AaWRKY1-overexpressing A. annua plants. These results showed that AaWRKY1 increased the content of artemisinin by regulating the expression of both ADS and CYP. It provides a new insight into the mechanism of regulation on artemisinin biosynthesis via transcription factors in the future.
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