ReviewCommunicative & integrative biology
Ultrasensitive detection of proteins and sugars at single-cell level.
Review in Communicative & integrative biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Modified ELISA for Ultrasensitive Diagnosis.Journal of clinical medicine · 2021Review
- Zeptomole Detection of an Enzyme by a Simple Colorimetric Method.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Antigen tests for COVID-19.Biophysics and physicobiology · 2021Article
- A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity.EBioMedicine · 2020Article
- Proposal of De Novo Antigen Test for COVID-19: Ultrasensitive Detection of Spike Proteins of SARS-CoV-2.Diagnostics (Basel, Switzerland) · 2020Article
- Experimental models and methods for cutaneous wound healing assessment.International journal of experimental pathology · 2020Review
- Ultrasensitive ELISA Developed for Diagnosis.Diagnostics (Basel, Switzerland) · 2019Review
- Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy.BMJ open diabetes research & care · 2019Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Each cell produces its own responses even if it appears identical to other cells. To analyze these individual cell characteristics, we need to measure trace amounts of molecules in a single cell. Nucleic acids in a single cell can be easily amplified by polymerase chain reaction, but single-cell measurement of proteins and sugars will require de novo techniques. In the present study, we outline the techniques we have developed toward this end. For proteins, our ultrasensitive enzyme-linked immunosorbent assay (ELISA) coupled with thionicotinamide-adenine dinucleotide cycling can detect proteins at subattomoles per assay. For sugars, fluorescence correlation spectroscopy coupled with glucose oxidase-catalyzed reaction allows us to measure glucose at tens of nM. Our methods thus offer versatile techniques for single-cell-level analyses, and they are hoped to strongly promote single-cell biology as well as to develop noninvasive tests in clinical medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.