ArticleScientific reports2016
Engineering formation of multiple recombinant Eut protein nanocompartments in E. coli.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Molecular basis of the biogenesis of a protein organelle for ethanolamine utilization.Science advances · 2025Article
- A blueprint for biomolecular condensation driven by bacterial microcompartment encapsulation peptides.Nature communications · 2025Article
- Article
- Bacterial microcompartment utilization in the human commensalJournal of bacteriology · 2024Article
- Ethanolamine-induced assembly of microcompartments is required forbioRxiv : the preprint server for biology · 2024Article
- IntegrativemSystems · 2024Article
- Role of ethanolamine utilization and bacterial microcompartment formation inInfection and immunity · 2024Article
- Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting.ACS nano · 2024Article
- Heterologous Assembly of Pleomorphic Bacterial Microcompartment Shell Architectures Spanning the Nano- to Microscale.Advanced materials (Deerfield Beach, Fla.) · 2023Article
- Engineering Bacillus subtilis for the formation of a durable living biocomposite material.Nature communications · 2021Article
- Protein scaffolds: A tool for multi-enzyme assembly.Biotechnology reports (Amsterdam, Netherlands) · 2021Review
- Advances in the World of Bacterial Microcompartments.Trends in biochemical sciences · 2021Review
- Prokaryotic Organelles: Bacterial Microcompartments inEcoSal Plus · 2020Review
- Genetic Characterization of a Glycyl Radical Microcompartment Used for 1,2-Propanediol Fermentation by Uropathogenic Escherichia coli CFT073.Journal of bacteriology · 2020Article
- Enzyme Assembly for Compartmentalized Metabolic Flux Control.Metabolites · 2020Review
- Engineering the PduT shell protein to modify the permeability of the 1,2-propanediol microcompartment ofMicrobiology (Reading, England) · 2019Article
- Supercharging enables organized assembly of synthetic biomolecules.Nature chemistry · 2019Article
- Alternative Pathways of Acetogenic Ethanol and Methanol Degradation in the Thermophilic AnaerobeFrontiers in microbiology · 2019Article
- Engineering nanoreactors using bacterial microcompartment architectures.Current opinion in biotechnology · 2018Review
- Bacterial encapsulins as orthogonal compartments for mammalian cell engineering.Nature communications · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compartmentalization of designed metabolic pathways within protein based nanocompartments has the potential to increase reaction efficiency in multi-step biosynthetic reactions. We previously demonstrated proof-of-concept of this aim by targeting a functional enzyme to single cellular protein nanocompartments, which were formed upon recombinant expression of the Salmonella enterica LT2 ethanolamine utilization bacterial microcompartment shell proteins EutS or EutSMNLK in Escherichia coli. To optimize this system, increasing overall encapsulated enzyme reaction efficiency, factor(s) required for the production of more than one nanocompartment per cell must be identified. In this work we report that the cupin domain protein EutQ is required for assembly of more than one nanocompartment per cell. Overexpression of EutQ results in multiple nanocompartment assembly in our recombinant system. EutQ specifically interacts with the shell protein EutM in vitro via electrostatic interactions with the putative cytosolic face of EutM. These findings lead to the theory that EutQ could facilitate multiple nanocompartment biogenesis by serving as an assembly hub for shell proteins. This work offers insights into the biogenesis of Eut bacterial microcompartments, and also provides an improved platform for the production of protein based nanocompartments for targeted encapsulation of enzyme pathways.
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