ArticleApplied microbiology and biotechnology2016
Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B.
Article in Applied microbiology and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Annexing isothermal nucleotide amplification enables probe-guided amplification and direct detection at ambient temperature.Nature communications · 2026Article
- Comparison of Different Reverse Transcriptase-Polymerase Chain Reaction-Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study.JMIR public health and surveillance · 2024Article
- Lateral Flow Biosensor for On-Site Multiplex Detection of Viruses Based on One-Step Reverse Transcription and Strand Displacement Amplification.Biosensors · 2024Article
- Manually pressurized droplet digital PCR chip for rapid SARS-CoV-2 diagnostics.Biomicrofluidics · 2024Article
- SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument.Scientific reports · 2021Article
- Influenza colloidal gold method and blood routine tests combination for rapid diagnosis of influenza: a decision tree-based analysis.NPJ primary care respiratory medicine · 2021Article
- Current and Future Point-of-Care Tests for Emerging and New Respiratory Viruses and Future Perspectives.Frontiers in cellular and infection microbiology · 2020Review
- Detection of human rhinoviruses by reverse transcription strand invasion based amplification method (RT-SIBA).Journal of virological methods · 2019Article
- Review
- Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay.Molecular diagnosis & therapy · 2018Article
- Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens.Diagnostic microbiology and infectious disease · 2018Article
- Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®.BMC infectious diseases · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and accurate diagnosis of influenza viruses plays an important role in infection control, as well as in preventing the misuse of antibiotics. Isothermal nucleic acid amplification methods offer significant advantages over the polymerase chain reaction (PCR), since they are more rapid and do not require the sophisticated instruments needed for thermal cycling. We previously described a novel isothermal nucleic acid amplification method, 'Strand Invasion Based Amplification' (SIBA®), with high analytical sensitivity and specificity, for the detection of DNA. In this study, we describe the development of a variant of the SIBA method, namely, reverse transcription SIBA (RT-SIBA), for the rapid detection of viral RNA targets. The RT-SIBA method includes a reverse transcriptase enzyme that allows one-step reverse transcription of RNA to complementary DNA (cDNA) and simultaneous amplification and detection of the cDNA by SIBA under isothermal reaction conditions. The RT-SIBA method was found to be more sensitive than PCR for the detection of influenza A and B and could detect 100 copies of influenza RNA within 15 min. The development of RT-SIBA will enable rapid and accurate diagnosis of viral RNA targets within point-of-care or central laboratory settings.
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