ArticleScientific reports2016
Generation of Hprt-disrupted rat through mouse←rat ES chimeras.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- One-Carbon Metabolism and Midbrain Dopaminergic Cells in Lesch-Nyhan Disease.Molecular syndromology · 2026Review
- Rat cell-derived kidney generation via interspecies blastocyst complementation in an Osr1-KO mouse model.Stem cell reports · 2026Article
- African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation.Cell reports methods · 2026Article
- Blastocyst complementation-based rat-derived heart generation reveals cardiac anomaly barriers to interspecies chimera development.iScience · 2024Article
- Generating an organ-deficient animal model using a multi-targeted CRISPR-Cas9 system.Scientific reports · 2024Article
- Article
- Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells.Stem cell reports · 2022Article
- Organotypic Rat Testicular Organoids for the Study of Testicular Maturation and Toxicology.Frontiers in endocrinology · 2022Article
- The road to generating transplantable organs: from blastocyst complementation to interspecies chimeras.Development (Cambridge, England) · 2021Article
- Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.Nature communications · 2021Article
- Lesch-Nyhan Syndrome: Models, Theories, and Therapies.Molecular syndromology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse←rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). In the present paper, we disrupted rat hypoxanthine-guanine phosphoribosyl transferase (Hprt) gene in ES cells and produced a Hprt-disrupted rat line using the mouse←rat ES chimera system. The mouse←rat ES chimera system demonstrated the dual advantages of space conservation and a clear indication of germ line transmission in knockout rat production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.