Evidence map›Paper›PMID 27062599›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Measuring Redox Status of Melanoma Cells.

Feng Liu-Smith, Tatiana B Krasieva, Jing Liu, Jiankang Liu, Frank L Meyskens

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Autophagy: In the cROSshairs of cancer.Biochemical pharmacology · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Feng Liu-SmithDepartment of Epidemiology, Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, B200 Sprague Hall, 839 Health Science Road, Irvine, CA, 92697, USA. liufe@uci.edu.
Tatiana B KrasievaBeckman Laser Institute and Medical Clinic, University of California Irvine, Irvine, CA, 92697, USA.
Jing LiuThe State Key Laboratory of Medical Genetics and School of Life Sciences, Central South University, Changsha, Hunan Province, China.
Jiankang LiuInstitute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University School of Life Science and Technology, Xi'an, Shaangxi Province, China.
Frank L MeyskensDepartment of Medicine, Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, B200 Sprague Hall, 839 Health Science Road, Irvine, CA, 92697, USA.

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Christine Hui · 1994 to 2026
$57.9M
CSUF and UCI-CFCCC Partnership for Cancer Health Disparities Research (2 of 2)P20CA174188 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI HUBBELL, FLOYD ALLAN · 2012 to 2015
$583k
NCI NIH HHS P20 CA174188NCI NIH HHS P30 CA062203
6 · The paper itself

Abstract

Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.

Indexed as

Dichlorofluorescin diacetate (DCFDA)Dihydroethidium (DHE)FluorometerHydrogen peroxideLaser microscopyReactive oxygen speciesRedox-sensitive GFPro1GFPro2GFPSuperoxide

Identifiers

PMID27062599
PMCPMC5878701

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.