Evidence map›Paper›PMID 27062568›Full record

ArticleCancer biomarkers : section A of Disease markers2016

Optimizing preservation of extracellular vesicular miRNAs derived from clinical cerebrospinal fluid.

Johnny C Akers, Valya Ramakrishnan, Isaac Yang, Wei Hua, Ying Mao, Bob S Carter, Clark C Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer biomarkers : section A of Disease markers, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed, 3 pooled it
4.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 3 syntheses or guidelines pooled it, 100 citations in OpenAlex.

  1. Pooled it
  2. Role of Exosomes in Dermal Wound Healing: A Systematic Review.The Journal of investigative dermatology · 2022
    Pooled it
  3. Pleiotropic Effects of Exosomes as a Therapy for Stroke Recovery.International journal of molecular sciences · 2020
    Pooled it
  4. Article
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  13. [Research advances on the role of microRNA engineered exosomes in diabetic wounds].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2024
    Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Extracellular Vesicles as Therapeutic Resources in the Clinical Environment.International journal of molecular sciences · 2023
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Johnny C AkersCenter for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
Valya RamakrishnanCenter for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
Isaac YangDepartment of Neurosurgery, University of California, Los Angeles, CA, USA.
Wei HuaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
Ying MaoDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
Bob S CarterCenter for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
Clark C ChenCenter for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
University of California, San Diego · USHuashan Hospital · CNCenter for Theoretical Biological Physics · USUniversity of California, Los Angeles · US

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DIANE M SIMEONE · 1985 to 2026
$124.9M
exRNA Biomarkers for Human GliomaUH3TR000931 · NCATS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CARTER, BOB S, HOCHBERG, FRED H · 2015 to 2017
$3.0M
exRNA Biomarkers for Human GliomaUH2TR000931 · NCATS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CARTER, BOB S, HOCHBERG, FRED H · 2013 to 2014
$1.1M
NCATS NIH HHS UH2 TR000931NCATS NIH HHS UH3 TR000931NCI NIH HHS P30 CA023100
6 · The paper itself

Abstract

backgroundTumor specific genetic material can be detected in extracellular vesicles (EVs) isolated from blood, cerebrospinal fluid (CSF), and other biofluids of glioblastoma patients. As such, EVs have emerged as a promising platform for biomarker discovery. However, the optimal procedure to transport clinical EV samples remains poorly characterized.

methodsWe examined the stability of EVs isolated from CSF of glioblastoma patients that were stored under different conditions. EV recovery was determined by Nanoparticle tracking analysis, and qRT-PCR was performed to determine the levels of miRNAs.

resultsCSF EVs that were lyophilized and stored at room temperature (RT) for seven days exhibited a 37-43% reduction in EV number. This reduction was further associated with decreased abundance of representative miRNAs. In contrast, the EV number and morphology remained largely unchanged if CSF were stored at RT. Total RNA and representative miRNA levels were well-preserved under this condition for up to seven days. A single cycle of freezing and thawing did not significantly alter EV number, morphology, RNA content, or miRNA levels. However, incremental decreases in these parameters were observed after two cycles of freezing and thawing.

conclusionsThese results suggest that EVs in CSF are stable at RT for at least seven days. Repeated cycles of freezing/thawing should be avoided to minimize experimental artifacts.

Indexed as

CryopreservationExtracellular VesiclesGene DosageHumansMicroRNAsRNA StabilityMicroRNAsCSFEVexosomefreeze thawlyophilizationstability

Identifiers

PMID27062568
PMCPMC4992418
OpenAlexW2324810994

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.