ArticleJournal of neurochemistry2016
Limited predictability of postmortem human brain tissue quality by RNA integrity numbers.
Article in Journal of neurochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
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- Integrity assay for messenger RNA in mouse and human brain samples and synaptosomal preparations.iScience · 2024Article
- Cell-type-specific effects of autism-associated 15q duplication syndrome in the human brain.American journal of human genetics · 2024Article
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- Altered Gene Expression Within the Renin-Angiotensin System in Normal Aging and Dementia.The journals of gerontology. Series A, Biological sciences and medical sciences · 2024Article
- Impact of fixation duration on messenger RNA detectability in human formalin-fixed paraffin-embedded brain tissue.Brain communications · 2024Article
- Different MAPT haplotypes influence expression of total MAPT in postmortem brain tissue.Acta neuropathologica communications · 2023Article
- Isolating mineralized bone and bone marrow mRNA from transiliac bone biopsies stored in a stabilizing solution: A comparative study.Bone reports · 2022Article
- RNA isolation from micro-quantity of articular cartilage for quantitative gene expression by microarray analysis.International journal of medical sciences · 2022Article
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- Optimization and evaluation of fluorescence in situ hybridization chain reaction in cleared fresh-frozen brain tissues.Brain structure & function · 2021Article
- Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing.BMC genomics · 2021Article
- Upregulation of Local Hepcidin Contributes to Iron Accumulation in Alzheimer's Disease Brains.Journal of Alzheimer's disease : JAD · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The RNA integrity number (RIN) is often considered to be a critical measure of the quality of postmortem human brains. However, it has been suggested that RINs do not necessarily reflect the availability of intact mRNA. Using the Agilent bioanalyzer and qRT-PCR, we explored whether RINs provide a meaningful way of assessing mRNA degradation and integrity in human brain samples by evaluating the expression of 3'-5' mRNA sequences of the cytochrome C-1 (CYC1) gene. Analysis of electropherograms showed that RINs were not consistently correlated with RNA or cDNA profiles and appeared to be poor predictors of overall cDNA quality. Cycle thresholds from qRT-PCR analysis to quantify the amount of CYC1 mRNA revealed positive correlations of RINs with amplification of full-length transcripts, despite the variable degree of linear degradation along the 3'-5' sequence. These data demonstrate that in postmortem human brain tissue the RIN is an indicator of mRNA quantity independent of degradation, but does not predict mRNA integrity, suggesting that RINs provide an incomplete measure of brain tissue quality. Quality assessment of postmortem human brains by RNA integrity numbers (RINs) may be misleading, as they do not measure intact mRNAs. We show that the RIN is an indicator of mRNA quantity independent of degradation, but does not predict mRNA integrity, suggesting that RINs provide an incomplete measure of brain tissue quality. Our results resolve controversial assumption on interpreting quality assessments of human postmortem brains by RINs.
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