ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2016
Heparin reduces nonspecific eosinophil staining artifacts in mass cytometry experiments.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 73 citations in OpenAlex.
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- Single-cell Unperturbed Activation Profiling by mass cytometry capturesFrontiers in immunology · 2026Article
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- Investigating immune profile by CyTOF in patients with eosinophilic esophagitis after treatment with orodispersible budesonide.Clinical and experimental immunology · 2024Article
- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Targeting AXL cellular networks in kidney fibrosis.Frontiers in immunology · 2024Article
- Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology.Nature immunology · 2023Article
- Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline.STAR protocols · 2022Article
- Combining Isoprenoid Probes with Antibody Markers for Mass Cytometric Analysis of Prenylation in Single Cells.Analytical chemistry · 2022Article
- Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV.Cell reports methods · 2022Article
- Optimization of methods for the accurate characterization of whole blood neutrophils.Scientific reports · 2022Article
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- In-Depth Immunophenotyping With Mass Cytometry During TB Treatment Reveals New T-Cell Subsets Associated With Culture Conversion.Frontiers in immunology · 2022Article
- Prolonged activation of nasal immune cell populations and development of tissue-resident SARS-CoV-2-specific CD8Nature immunology · 2022Observational
- CyTOFFrontiers in immunology · 2022Review
- T lymphocyte and monocyte subsets are dysregulated in type 1 diabetes patients with peripheral neuropathic pain.Brain, behavior, & immunity - health · 2021Article
- A streamlined whole blood CyTOF workflow defines a circulating immune cell signature of COVID-19.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The analysis of heterogeneous cell samples by mass cytometry (CyTOF) relies on the assumption that metal labeled antibodies accurately bind to their target antigens. We report a previously unappreciated experimental artifact of non-specific antibody binding by eosinophils during intracellular CyTOF analysis of human whole blood samples. We hypothesized that this non-specific binding results from a charge-based interaction between the metal-labeled antibodies and highly cationic proteins found in eosinophillic granules and found that this non-specific staining artifact could be reduced to background levels with a simple blocking protocol using heparin as a competing anionic protein. This protocol eliminates a potential source of erroneous data interpretation in all experiments involving intracellular staining of human whole blood samples, and allows accurate assessment of dynamic changes in intracellular proteins in eosinophils by CyTOF. © 2016 International Society for Advancement of Cytometry.
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