ArticleMetabolic engineering2016
Extreme promiscuity of a bacterial and a plant diterpene synthase enables combinatorial biosynthesis.
Article in Metabolic engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 68 citations in OpenAlex.
- Characterization of two key cytochrome P450 enzymes inSynthetic and systems biotechnology · 2027Article
- Exploring a Unique Class II Diterpene Cyclase: The Modified Catalytic Acid Motif Contributes to Ring Contraction in Premutilin Synthase.Organic letters · 2026Article
- Determining the Antimycobacterial Action of Rottlerin Against Mycobacterium Species and Toxicity, Antioxidant Properties, and Therapeutic Target Affinity of Rottlerin.Current microbiology · 2025Article
- Chemical and transcriptomic analyses of leaf trichomes from Cistus creticus subsp. creticus reveal the biosynthetic pathways of certain labdane-type diterpenoids and their acetylated forms.Journal of experimental botany · 2024Article
- From functional plasticity of two diterpene synthases (IrTPS2/IrKSL3a) to enzyme evolution.ACS catalysis · 2024Article
- Sequence-Structure Analysis Unlocking the Potential Functional Application of the Local 3D Motifs of Plant-Derived Diterpene Synthases.Biomolecules · 2024Article
- Mechanistic docking in terpene synthases using EnzyDock.Methods in enzymology · 2024Review
- Structural and functional investigations of syn-copalyl diphosphate synthase from Oryza sativa.Communications chemistry · 2023Article
- Product Profiles of Promiscuous Enzymes Can be Altered by Controlling In Vivo Spatial Organization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Plant (di)terpenoid evolution: from pigments to hormones and beyond.Natural product reports · 2023Review
- Investigation of Acid-Base Catalysis in Halimadienyl Diphosphate Synthase Involved inACS bio & med chem Au · 2022Article
- Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks.Nature communications · 2022Article
- Diterpene synthases from Leonurus japonicus elucidate epoxy-bridge formation of spiro-labdane diterpenoids.Plant physiology · 2022Article
- Tanshinones: Leading the way into Lamiaceae labdane-related diterpenoid biosynthesis.Current opinion in plant biology · 2022Review
- Functional identification of the terpene synthase family involved in diterpenoid alkaloids biosynthesis inActa pharmaceutica Sinica. B · 2021Article
- Magnesium-specific ring expansion/contraction catalysed by the class II diterpene cyclase from pleuromutilin biosynthesis.Organic & biomolecular chemistry · 2020Article
- Antibacterial Natural Halimanes: Potential Source of Novel Antibiofilm Agents.Molecules (Basel, Switzerland) · 2020Review
- Nerylneryl diphosphate is the precursor of serrulatane, viscidane and cembrane-type diterpenoids in Eremophila species.BMC plant biology · 2020Article
- Genomics-enabled analysis of specialized metabolism in bioenergy crops: current progress and challenges.Synthetic biology (Oxford, England) · 2020Review
- Correction: cis or trans with class II diterpene cyclases.Organic & biomolecular chemistry · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Diterpenes are widely distributed across many biological kingdoms, where they serve a diverse range of physiological functions, and some have significant industrial utility. Their biosynthesis involves class I diterpene synthases (DTSs), whose activity can be preceded by that of class II diterpene cyclases (DTCs). Here, a modular metabolic engineering system was used to examine the promiscuity of DTSs. Strikingly, both a bacterial and plant DTS were found to exhibit extreme promiscuity, reacting with all available precursors with orthogonal activity, producing an olefin or hydroxyl group, respectively. Such DTS promiscuity enables combinatorial biosynthesis, with remarkably high yields for these unoptimized non-native enzymatic combinations (up to 15mg/L). Indeed, it was possible to readily characterize the 13 unknown products. Notably, 16 of the observed diterpenes were previously inaccessible, and these results provide biosynthetic routes that are further expected to enable assembly of more extended pathways to produce additionally elaborated 'non-natural' diterpenoids.
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