ArticleGenesis (New York, N.Y. : 2000)2016
Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins.
Article in Genesis (New York, N.Y. : 2000), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- A guide to cell-type-specific Cre mouse models in atherosclerosis: from background strains to future directions.Frontiers in cardiovascular medicine · 2026Review
- Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor.Cell stem cell · 2024Article
- Identification of distinct vascular mural cell populations during zebrafish embryonic development.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Alkali injury-induced pathological lymphangiogenesis in the iris facilitates the infiltration of T cells and ocular inflammation.JCI insight · 2024Article
- Stretch regulates alveologenesis and homeostasis via mesenchymal Gαq/11-mediated TGFβ2 activation.Development (Cambridge, England) · 2023Article
- The ebb and flow of cardiac lymphatics: a tidal wave of new discoveries.Physiological reviews · 2023Review
- PDGFRβCell reports · 2022Article
- Emerging roles for CNS fibroblasts in health, injury and disease.Nature reviews. Neuroscience · 2022Review
- Review
- Patterns of differentiation of renin lineage cells during nephrogenesis.American journal of physiology. Renal physiology · 2021Article
- Mechanisms and cell lineages in lymphatic vascular development.Angiogenesis · 2021Review
- The evolving cardiac lymphatic vasculature in development, repair and regeneration.Nature reviews. Cardiology · 2021Review
- Formation and Growth of Cardiac Lymphatics during Embryonic Development, Heart Regeneration, and Disease.Cold Spring Harbor perspectives in biology · 2020Review
- A Second Heart Field-Derived Vasculogenic Niche Contributes to Cardiac Lymphatics.Developmental cell · 2020Article
- Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D.Frontiers in physiology · 2020Article
- Development of mouse models of angiosarcoma driven by p53.Disease models & mechanisms · 2019Article
- The Cdh5-CreERT2 transgene causes conditional Shb gene deletion in hematopoietic cells with consequences for immune cell responses to tumors.Scientific reports · 2019Article
- Zebrafish facial lymphatics develop through sequential addition of venous and non-venous progenitors.EMBO reports · 2019Article
- Cardiac lymphatics in health and disease.Nature reviews. Cardiology · 2019Review
- The deployment of cell lineages that form the mammalian heart.Nature reviews. Cardiology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Pdgfrb-Cre line has been used as a tool to specifically target pericytes and vascular smooth muscle cells. Recent studies showed additional targeting of cardiac and mesenteric lymphatic endothelial cells (LECs) by the Pdgfrb-Cre transgene. In the heart, this was suggested to provide evidence for a previously unknown nonvenous source of LECs originating from yolk sac (YS) hemogenic endothelium (HemEC). Here we show that Pdgfrb-Cre does not, however, target YS HemEC or YS-derived erythro-myeloid progenitors (EMPs). Instead, a high proportion of ECs in embryonic blood vessels of multiple organs, as well as venous-derived LECs were targeted. Assessment of temporal Cre activity using the R26-mTmG double reporter suggested recent occurrence of Pdgfrb-Cre recombination in both blood and lymphatic ECs. It thus cannot be excluded that Pdgfrb-Cre mediated targeting of LECs is due to de novo expression of the Pdgfrb-Cre transgene or their previously established venous endothelial origin. Importantly, Pdgfrb-Cre targeting of LECs does not provide evidence for YS HemEC origin of the lymphatic vasculature. Our results highlight the need for careful interpretation of lineage tracing using constitutive Cre lines that cannot discriminate active from historical expression. The early vascular targeting by the Pdgfrb-Cre also warrants consideration for its use in studies of mural cells. genesis 54:350-358, 2016. © 2016 The Authors. Genesis Published by Wiley Periodicals, Inc.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.