Evidence map›Paper›PMID 27060366›Full record

ArticleInternational forum of allergy & rhinology2016

Characterization of a novel high-dose ovalbumin-induced murine model of allergic sinonasal inflammation.

Michelle Mendiola, Anuj Tharakan, Mengfei Chen, Tomefa Asempa, Andrew P Lane, Murugappan Ramanathan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International forum of allergy & rhinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Airborne Particulate Matter Induces Nonallergic Eosinophilic Sinonasal Inflammation in Mice.American journal of respiratory cell and molecular biology · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Michelle MendiolaDivision of Rhinology and Sinus Surgery, Department of Otolaryngology-Head and Neck Surgery, Baltimore, MD.
Anuj TharakanDivision of Rhinology and Sinus Surgery, Department of Otolaryngology-Head and Neck Surgery, Baltimore, MD.
Mengfei ChenDivision of Rhinology and Sinus Surgery, Department of Otolaryngology-Head and Neck Surgery, Baltimore, MD.
Tomefa AsempaDivision of Rhinology and Sinus Surgery, Department of Otolaryngology-Head and Neck Surgery, Baltimore, MD.
Andrew P LaneDivision of Rhinology and Sinus Surgery, Department of Otolaryngology-Head and Neck Surgery, Baltimore, MD.
Murugappan RamanathanDivision of Rhinology and Sinus Surgery, Department of Otolaryngology-Head and Neck Surgery, Baltimore, MD. mramana3@jhmi.edu.
Sinai Hospital · US

Funding

Role of the Indoor Environment on Oxidative Stress Related Sinonasal InflammationK23ES020859 · NIEHS · JOHNS HOPKINS UNIVERSITY · PI RAMANATHAN, MURUGAPPAN · 2011 to 2015
$1.2M
NIEHS NIH HHS K23 ES020859NIEHS NIH HHS L30 ES022003
6 · The paper itself

Abstract

backgroundFew efficacious topical therapies exist for chronic rhinosinusitis (CRS). The lack of a reproducible mouse model of CRS limits the pilot testing of potential novel anti-inflammatory therapies. Although the ovalbumin-induced mouse model of sinonasal inflammation is commonly used, it is difficult to reproduce and can generate variable histologic results. In this study, we explore a variation of this model in different strains of mice and explore various inflammatory cytokines as reproducible molecular markers of inflammation.

methodsAllergic sinonasal inflammation was generated in BALB/c and C57BL/6 mice using intraperitoneal high-dose injections of ovalbumin (Ova; Sigma Chemical Co.) followed by 10 days of high-dose intranasal sensitization. Real-time polymerase chain reaction (RT-PCR) for eotaxin, interleukin 4 (IL-4), and IL-13 were measured from sinonasal mucosa. We also pilot tested a known topical budesonide to characterize the anti-inflammatory response. Histological sections were analyzed for epithelial thickness and eosinophilia.

resultsBoth BALB/c and C57BL/6 mice consistently showed increases in T helper 2 (Th2) cytokines after sensitization with high-dose Ova (p < 0.0001) when compared to controls. There were also significant increases in epithelial thickening in Ova-sensitized mice and eosinophilia in both BALB/c and C57BL/6 strains. In addition, topical budesonide significantly reduced anti-inflammatory cytokines, eosinophilia, and epithelial thickness.

conclusionOur variation of the ovalbumin-induced mouse model of sinonasal inflammation in both BALB/c and C57BL/6 mice provides an efficacious model for testing potential topical anti-inflammatory therapies for CRS. The utilization of sinonasal mucosal Th2 cytokines along with histologic markers provides a consistent and quantifiable marker of inflammation in assessing the efficacy of candidate drugs.

Indexed as

AllergensDisease Models, AnimalHypersensitivityOvalbuminSinusitisAnimalsAnti-Inflammatory AgentsBudesonideCytokinesEosinophiliaFemaleMaleMice, Inbred BALB CMice, Inbred C57BLNasal Lavage FluidNasal MucosaAllergensAnti-Inflammatory AgentsBudesonideCytokinesOvalbuminRNA, Messengerallergic rhinosinusitiseosinophiliamouse modelovalbumin

Identifiers

PMID27060366
PMCPMC5012925
OpenAlexW2339553150

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.